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Characterization of an immediate-early gene induced in adherent monocytes that encodes IκB-like activity

1991; Cell Press; Volume: 65; Issue: 7 Linguagem: Inglês

10.1016/0092-8674(91)90022-q

1097-4172

Stephen Haskill, Amer A. Beg, S. Mark Tompkins, John S Morris, Andrew D. Yurochko, Adam Sampson-Johannes, Krishna Mondal, Peter Ralph, Albert S. Baldwin,

RNA regulation and disease

We have cloned a group of cDNAs representing mRNAs that are rapidly induced following adherence of human monocytes. One of the induced transcripts (MAD-3) encodes a protein of 317 amino acids with one domain containing five tandem repeats of the cdc10/ankyrin motif, which is 60% similar (46% identical) to the ankyrin repeat region of the precursor of p50. The C-terminus has a putative protein kinase C phosphorylation site. In vitro translated MAD-3 protein was found to specifically inhibit the DNA-binding activity of the NF-κB complex but not that of the KBF1 factor or of other DNA-binding proteins. The MAD-3 cDNA encodes an IκB-like protein that is likely to be involved in regulation of transcriptional responses to NF-κB, including adhesion-dependent pathways of monocyte activation.