The in vivo induction of rat hepatic cytochrome P450-dependent enzyme activities and their maintenance in culture
1990; Elsevier BV; Volume: 40; Issue: 3 Linguagem: Inglês
10.1016/0006-2952(90)90567-5
ISSN1873-2968
AutoresAlison H. Hammond, Jeffrey R. Fry,
Tópico(s)Eicosanoids and Hypertension Pharmacology
ResumoCytochrome P450-dependent enzyme activities were measured in hepatocytes from adult male rats, induced in vivo with phenobarbitone, β-naphthoflavone, dexamethasone or isoniazid; the stability of the induced activities in culture was also determined. Each inducer produced a characteristically different pattern of enzyme activities with dexamethasone, isoniazid and β-naphthoflavone selectively inducing erythromycin N-demethylase, p-nitrophenol hydroxylase and ethoxyresorufin O-dealkylase respectively. In general, the induced activities were maintained for 24 hr in culture. This indicates the feasibility of an in vivo induction-hepatocyte culture system for the study of metabolism-mediated toxicity.
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