Portal de Periódicos da CAPES

Use of staphylococcal nuclease gene as DNA probe forStaphylococcus aureus

1987; Oxford University Press; Volume: 44; Issue: 2 Linguagem: Inglês

10.1111/j.1574-6968.1987.tb02264.x

1574-6968

Wolfgang Liebl, Ralf Rosenstein, Friedrich Götz, Karl‐Heinz Schleifer,

Antimicrobial Resistance in Staphylococcus

A 518-base pair fragment of the cloned gene coding for the thermostable nuclease of Staphylococcus aureus strain Foggi was used as a DNA probe for hybridization to DNA of various staphylococci. The strains were tested for their ability to produce DNase. All strains of S. aureus produced heat-stable DNase and their DNA reacted with the nuclease gene. Other staphylococci producing heat-stable DNase did not react in a dot hybridization with the nuclease gene probe.