Androgen biosynthesis by marmoset testes in vitro
1977; Elsevier BV; Volume: 31; Issue: 1 Linguagem: Inglês
10.1016/0016-6480(77)90196-4
ISSN1095-6840
AutoresJames P. Preslock, Emil Steinberger,
Tópico(s)Sexual Differentiation and Disorders
ResumoThese studies were undertaken to determine the major steroid metabolites formed from selected androgen precursors by the testis of the marmoset, Saguinus oedipus, a New World primate of the family Callitricadae. Testicular fragments (50 mg) were incubated for 3.0 hr with pregnenolone-7-3H or with progesterone-7-3H. The major metabolites formed from pregnenolone were 17α-hydroxyprogesterone (42.7%), testosterone (20.5%), androstenedione (11.4%) and progesterone (9.2%). Nonmetabolized substrate was 6.8% of radioactivity. For porgesterone incubations, 17α-hydroxyprogesterone was the major matabolite (49.0%), with testosterone (21.2%) and androstenedione (10.7%) as lesser metabolites. Unreacted progesterone accounted for 14.9% of all radioactivity. The unusually high levels of 17α-hydroxyprogesterone in marmosets is in contrast to that observed in other mammalian species.
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