Ovarian Follicular Development: From Physiology to Molecular Biology
1987; Elsevier BV; Linguagem: Inglês
10.1016/b978-0-12-571143-2.50012-5
AutoresJoAnne S. Richards, Tore Jahnsen, Lars O. Hedin, JAMI LIFKA, SHERI L. RATOOSH, JON M. DURICA, Noga B. Goldring,
Tópico(s)Ovarian function and disorders
ResumoTheca cell differentiation and the synthesis of aromatizable androgens are obligatory for follicular estradiol production and the differentiation of granulosa cells. Previous studies indicated that theca cell differentiation was dependent on subtle increases in serum luteinizing hormone (LH) concentrations. To determine whether the effects of LH on theca cell 17 α-hydroxylase in vivo were mediated by cAMP, a theca cell culture system using theca explants from small antral (SA), preovulatory (PO), and luteinizing follicles was developed. SA theca produces only low amounts of androstenedione when cultured in medium alone. However, either when LH was added at the beginning of culture or when forskolin was added on day 4 of culture androstenedione, production was increased to 5–10 ng ml/theca and was maintained for 20 days of culture. PO theca initially produced more androstenedione than SA theca but failed to maintain steroid synthesis unless either LH or forskolin was added to the cultures.
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