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To pool or not to pool? Impact of the use of individual and pooled fecal samples for in vitro fermentation studies

2014; Elsevier BV; Volume: 107; Linguagem: Inglês

10.1016/j.mimet.2014.08.022

1872-8359

Marisol Aguirre, Javier Ramiro-García, M. Koenen, Koen Venema,

Clostridium difficile and Clostridium perfringens research

This study investigated the stability and the activity of the microbiota from a single and a pool of donors in the TNO in vitro model of the colon (TIM-2 system). Our findings demonstrate the suitability of the preparation of a pool of fecal sample to be used for fermentation experiments.