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The Oxygen-Rich Postnatal Environment Induces Cardiomyocyte Cell-Cycle Arrest through DNA Damage Response

2014; Cell Press; Volume: 157; Issue: 3 Linguagem: Inglês

10.1016/j.cell.2014.03.032

1097-4172

Bao Nguyen Puente, Wataru Kimura, Shalini Muralidhar, Jesung Moon, James F. Amatruda, Kate L. Phelps, D. Bennett Grinsfelder, Beverly A. Rothermel, Rui Chen, Joseph A. Garcia, Célio X.C. Santos, Suwannee Thet, Eiichiro Mori, Michael Kinter, Paul M. Rindler, Serena Zacchigna, Shibani Mukherjee, David J. Chen, Ahmed I. Mahmoud, Mauro Giacca, Peter S. Rabinovitch, Aroumougame Asaithamby, Ajay M. Shah, Luke I. Szweda, Hesham A. Sadek,

Tissue Engineering and Regenerative Medicine

The mammalian heart has a remarkable regenerative capacity for a short period of time after birth, after which the majority of cardiomyocytes permanently exit cell cycle. We sought to determine the primary postnatal event that results in cardiomyocyte cell-cycle arrest. We hypothesized that transition to the oxygen-rich postnatal environment is the upstream signal that results in cell-cycle arrest of cardiomyocytes. Here, we show that reactive oxygen species (ROS), oxidative DNA damage, and DNA damage response (DDR) markers significantly increase in the heart during the first postnatal week. Intriguingly, postnatal hypoxemia, ROS scavenging, or inhibition of DDR all prolong the postnatal proliferative window of cardiomyocytes, whereas hyperoxemia and ROS generators shorten it. These findings uncover a protective mechanism that mediates cardiomyocyte cell-cycle arrest in exchange for utilization of oxygen-dependent aerobic metabolism. Reduction of mitochondrial-dependent oxidative stress should be an important component of cardiomyocyte proliferation-based therapeutic approaches.