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Chronic transfusion practices for prevention of primary stroke in children with sickle cell anemia and abnormal TCD velocities

2011; Wiley; Volume: 87; Issue: 4 Linguagem: Inglês

10.1002/ajh.23105

1096-8652

Banu Aygün, Lisa Wruck, William H. Schultz, Brigitta U. Mueller, R. Clark Brown, Lori Luchtman‐Jones, Sherron M. Jackson, Rathi V. Iyer, Zora R. Rogers, Sharada A. Sarnaik, Alexis A. Thompson, Cynthia Gauger, Ronald W. Helms, Russell E. Ware, Brigitta U. Mueller, Bogdan Dinu, Kusum Viswanathan, Natalie Sommerville-Brooks, Betsy Record, Matthew M. Heeney, Meredith Anderson, Janet L. Kwiatkowski, J. R. Olson, M.C. Brown, Lakshmanan Krishnamurti, R. W. McCollum, Kamar Godder, Jennifer Newlin, William Owen, Stephen C. Nelson, Katie Bianchi, Sheronda Brown, Margaret Lee, Courtney D. Thornburg, Charles Daeschner, Cynthia Brown, Lisa Kuisel, Ramamoorthy Nagasubramanian, Brian Berman, Mary DeBarr, Sharon Singh, Antonella Farrell, Eileen Hansbury, Scott Miller, Kathy Rey, Isaac Odame, Nagina Parmar, Manuella Merelles-Pulcinni, Leah Adix, Lee Hilliard, Jeanine Dumas, Michelle Neier, Stephanie Ferreira de Farias, Alvarez Ofelia Alvarez, Williams Patrice Williams, Mary T. Walker, Hamayun Imran, Stephanie Durggin, Elizabeth Yang, Mary Murphy,

Iron Metabolism and Disorders

Chronic transfusions are recommended for children with sickle cell anemia (SCA) and abnormal transcranial Doppler (TCD) velocities (≥ 200 cm/sec) to help prevent the occurrence of a primary stroke [1]. The goal is usually to maintain the sickle hemoglobin concentration (HbS) <30%; however, this goal is often difficult to achieve in clinical practice. The NHLBI-sponsored trial "TCD With Transfusions Changing to Hydroxyurea (TWiTCH)" will compare standard therapy (transfusions) to alternative therapy (hydroxyurea) for the reduction of primary stroke risk in this patient population. Transfusions will be given according to current transfusion practices at participating sites. To determine current academic community standards for primary stroke prophylaxis in children with SCA, 32 clinical sites collected data on 340 children with abnormal TCD velocities receiving chronic transfusions to help prevent primary stroke. The average (mean ± 1 SD) pretransfusion HbS was 34 ± 11% (institutional average 23–48%); the 75th and 90th percentiles were 41 and 50%, respectively. Lower %HbS was associated with higher pretransfusion Hb values and receiving transfusions on time. These data indicate variable current transfusion practices among academic pediatric institutions and in practice, 30% HbS may not be an easily attainable goal in this cohort of children with SCA and abnormal TCD. Children with sickle cell anemia (SCA) compose a high risk group for the development of stroke. If untreated, 11% will experience a clinical stroke by 20 years of age [2]. Adams et al. have shown that children with SCA who are at risk for primary stroke can be identified by measuring time-averaged mean blood flow velocities in the internal carotid or middle cerebral arteries by TCD [3]. Abnormal TCD velocities (≥200 cm/sec) are associated with high risk for stroke and warrant transfusion therapy to reduce the risk of primary stroke. First stroke can be successfully prevented in 90% of children with SCA and abnormal TCD velocities by the use of chronic transfusion therapy, with a goal of keeping HbS concentrations less than 30% [1]. TCD with Transfusions Changing to Hydroxyurea (TWiTCH) is an NHLBI-sponsored, Phase III, multicenter trial comparing standard therapy (monthly transfusions) to alternative therapy (daily hydroxyurea) to reduce the risk of primary stroke in children with SCA and documented abnormal TCD velocities. Since transfusions compose the standard treatment arm, accurate %HbS values achieved in actual clinical practice were needed for protocol development. The majority of our information about transfusing patients with SCA to prevent stroke comes from secondary stroke prevention, i.e., the use of chronic red blood cell transfusions to prevent a second strokeafter a first clinical stroke has occurred. Classically, transfusions are administered at 4-week intervals to maintain HbS at less than 30%. After several years of transfusion therapy, a few centers increase transfusion interval to 5–6 weeks and allow HbS to increase toward 50% in selected patients [4, 5]. Our previous study in 295 children with SCA who received transfusions for secondary stroke prevention revealed an average pretransfusion HbS of 35 ± 11% with highly variable institutional %HbS levels ranging from 22 to 51% [6] In order to determine the current clinical standard of transfusion therapy for primary stroke prevention for elevated TCD velocities, we performed a larger survey of potential TWiTCH sites. We hypothesized that average pretransfusion HbS values achieved at pediatric academic centers would be higher than 30%. This study defines the current practice at academic medical centers in provision of chronic transfusion therapy to help reduce the risk of primary stroke in children with SCA. A total of 340 children with SCA and history of abnormal TCD velocities receiving chronic PRBC transfusions for primary stroke prophylaxis were identified at 32 institutions (Table I). The number of patients per site ranged from 3 to 33 (median 9 per site). A total of 3,970 transfusions were administered over the 12-month period, with a mean of 11.7 ± 2.8 transfusions per patient. Results were similar when analyzed by each patient contributing equally or each transfusion contributing equally (Table II). The predominant transfusion type by patient was defined as the technique used ≥6 times over the 12-month period. Most children (79%) received primarily simple transfusions, while 19% had primarily exchange transfusions (11% partial /manual exchange, 8% erythrocytapheresis), and 2% multiple transfusion types. The transfusion goal was <30% at almost all sites (84%), while at five sites, the %HbS was allowed in selected patients to increase to 50% after a period of clinical stability. The majority (95%) of the transfusions were administered within the defined 7-day window. On average, late transfusions were given 1.3 ± 5.5 days after the defined 7-day window. Thirty percent of the patients had at least one late transfusion and 14% had 2 or more late transfusions in the 1-year period. For the 3,653 transfusions with reported %HbS values (representing 92% of the 3,970 transfusions), the mean pretransfusion HbS percentage was 33.2 ± 14.0% (median 32%). The 75th percentile for HbS values was 41%, while the 90th percentile was 51%. There were substantial differences among institutional pretransfusion %HbS values, ranging from 23 ± 14% HbS at one institution where HbS was reported for 103 transfusions given to nine patients during the 12-month period, to 48 ± 15% at another institution where HbS was reported for 95 transfusions administered to nine patients during the same time frame (Table III). The five sites with increased HbS goals to 50% in selected patients did not have higher values than others. For each transfusion, subjects were less likely to have pretransfusion HbS <30% if they were older [OR 0.92 for each year increase in age, 95% CI (0.89, 0.96)] and on transfusions for a longer period of time [OR 0.90 for each year increase in duration, 95% CI (0.86, 0.94)]. Patients with higher pretransfusion Hb levels were more likely to have pretransfusion HbS <30% [OR 1.63 for each g/dL increase in Hb, 95% CI (1.46, 1.83)] and late transfusions were less likely to be associated with a pretransfusion HbS <30% [OR 0.27, 95% CI (0.18, 0.41)]. The Hb result does not appear to be a function of late transfusions since both covariates remained significant when modeled jointly. History of allo- or autoantibodies, TCD velocity, and erythrocytapheresis use were not significant predictors of a pretransfusion HbS <30%. During the initial STOP study, transfusions were given to maintain pretransfusion HbS values at less than 30% [3]. However, there were frequent transient rises of HbS above this level [7]. Furthermore, extended follow-up results from the STOP study showed that pretransfusion %HbS values during the post-trial follow-up were higher than those during the STOP study [8]. The average %HbS per patient was 27.5 ± 12.4, still within the desired goal of 30%. However, pretransfusion HbS levels were 30–34.9% in 12%, 35–39.9% in 7%, and greater than 40% in 12% of the transfusions. In the STOP2 study, where children with abnormal TCD velocities whose Doppler readings became normal were randomly assigned to continue or stop transfusions, 24% of the patients had pretransfusion HbS levels greater than 30% [9]. These findings indicate that even in the context of a prospective clinical trial, maintaining HbS <30% was difficult to achieve. With the subsequent recommendation to treat all children with SCA who are at risk for primary stroke with transfusions to maintain HbS <30%, the feasibility of this approach in actual clinical practice is not known. Possible obstacles to achieving this goal include (1) physician and staff goals regarding TCD screening and chronic transfusion therapy; (2) family understanding of the need for on-time transfusions and other factors affecting patient adherence; (3) hypersplenism; (4) development of allo-and auto-antibodies; (5) difficulties with venous access. In a recent report describing the effects of chronic transfusion therapy on TCD velocities, the pretransfusion HbS levels for 88 children who were identified to have abnormal TCD velocities during screening for STOP2 was noted to be 37 ± 20% [10]. A single institution retrospective study from France involving 17 children with SCA with abnormal TCD velocities reported mean pretransfusion HbS levels were 30 ± 10% during chronic transfusion therapy, but with a follow-up period of only 39 patient-years [11]. We have conducted a similar survey analyzing chronic transfusion practice for 295 children with SCA and previous stroke at 23 US institutions [6]. Results of that study were very similar to the current study including an average pretransfusion %HbS of 35 ± 11%. Receiving scheduled transfusions on time was identified as the most significant factor to maintain HbS at ≤30%. Finally, in a prospective cohort study, the mean pretransfusion %HbS values of 39 children with SCA and stroke followed at seven academic centers was 26.9% (range, 3.94–48.3%) [12]. With a broad geographical distribution of 32 clinical sites and inclusion of all TWiTCH-eligible patients, our data provide an accurate "snapshot" of current therapy for children with SCA and abnormal TCD velocities receiving treatment at leading academic centers in North America. The mean age at start of chronic transfusions, frequency and duration of transfusions, compliance, and types of transfusions, and %HbS goals are similar to published reports and represent the clinical experience of pediatric hematologists at tertiary academic medical centers with large clinical transfusion programs. Importantly, %HbS was often not maintained at the accepted "gold standard" of <30%; the mean pretransfusion %HbS, calculated either by transfusion or by patient, was about 34%; the 75th and 90th percentiles were 41 and 50% HbS, respectively. Of all the variables studied, the only ones with a substantial impact on the probability of a transfusion achieving the 30% HbS goal were age of the patient, duration of transfusion therapy, pretransfusion Hb concentration, and whether transfusions were given on time. One limitation of this retrospective survey is information on other variables such as hypersplenism or infectious illnesses that may have caused deviations on %HbS levels was not collected. Of the variables analyzed, maintaining a higher Hb concentration and receiving transfusions on time appear to be the best ways to achieve the desired %HbS levels for children on chronic transfusion therapy. Deviations from accepted "gold standard" may change clinical outcomes such as TCD velocity reductions, change in cerebral vasculopathy, or stroke prevention. As a result of this analysis, the TWiTCH study will recommend chronic transfusions with the goal of maintaining HbS <30%, but only record a protocol violation when the HbS value exceeds 45%. After IRB approval at each institution, TWiTCH clinical investigators completed a transfusion survey for each potentially eligible TWiTCH participant at their site without including any protected health information. Eligibility criteria included the diagnosis of SCA, age 4.0–15.9 years, history of abnormal TCD velocities (≥200 cm/sec), and currently receiving chronic transfusion therapy to reduce the risk of primary stroke. Since protected health information was not collected, signed consent was typically not required. Participating clinical sites and personnel are listed in the Appendix. Subject and transfusion information were collected retrospectively for each patient over a 12-month period from September 1, 2008 to August 31, 2009. Subject data included year of birth, gender, the year chronic transfusions began, and TCD velocities that led to the start of transfusion therapy. Day and month of birth were not collected to ensure anonymized data. The transfusion goal for each patient (30% HbS versus 50% HbS) and a history of erythrocyte allo- and auto-antibodies were also recorded for each patient. Information collected from each transfusion during the 12-month period included the patient's weight (kg), pretransfusion hemoglobin concentration (Hb, gm/dL), pretransfusion %HbS, volume and type of transfusion (simple, partial exchange, erythrocytapheresis) and, and number of days beyond the scheduled date of transfusion. Children with SCA who are on chronic transfusion therapy usually receive a transfusion every 3–5 weeks to maintain the desired %HbS. Every subject is on an individual schedule based on his/her %HbS levels. For the purposes of this survey, a transfusion was defined as "late" if it was administered more than 7 days beyond the scheduled date. Categorical variables were summarized using frequency counts and percentages. Continuous variables were summarized using one or more of the following: mean, standard deviation, median, minimum, and maximum, 75th and 90th percentile, ignoring missing data when applicable. Age at survey, age at start of chronic transfusion therapy, average weight, average pretransfusion Hb and %HbS, average transfusion volume, duration of transfusion therapy and predominant transfusion type were summarized with each patient contributing equally. Weight, pretransfusion Hb and %HbS, transfusion volume, and days late were also summarized with each transfusion contributing equally. The probability of pretransfusion %HbS less than 30% was modeled using generalized estimating equations to account for the lack of independence induced by multiple transfusions per subject. The models were used to generate estimates of the odds ratio (OR) and 95% confidence intervals. Results were considered statistically significant if the confidence interval excluded one. The authors thank the entire TWiTCH Trial Group (members listed in "Appendix") and Rho Federal Systems Division, Inc. (Nancy Yovetich PhD, Christopher Woods, Jamie Spencer, Marsha McMurray) for their valuable contributions to the study. TWiTCH Trial investigators and key contributors include the following: Brigitta Mueller and Bogdan Dinu (Baylor College of Medicine, Houston, TX); Kusum Viswanathan and Natalie Sommerville-Brooks (Brookdale Hospital Medical Center, Brooklyn, NY); Clark Brown and Betsy Record (Children's Healthcare of Atlanta, Atlanta, GA); Matthew Heeney and Meredith Anderson (Children's Hospital Boston, Boston, MA); Janet L. Kwiatkowski, Jeffrey Olson and Martha Brown, (Children's Hospital of Philadelphia, Philadelphia, PA); Lakshmanan Krishnamurti and Regina McCollum (Children's Hospital of Pittsburgh, Pittsburgh, PA); Kamar Godder and Jennifer Newlin (Children's Hospital of Richmond, Richmond, VA); William Owen (Children's Hospital of the King's Daughters); Stephen Nelson (Children's Hospitals and Clinics of Minnesota, Minneapolis, MN); Alexis A. Thompson and Katie Bianchi (Children's Memorial Hospital, Chicago, IL); Lori Luchtman-Jones and Sheronda Brown (Children's National Medical Center, Washington, DC); Margaret Lee (Columbia University, New York, NY); Courtney Thornburg (Duke University Medical Center, Durham, NC); Charles Daeschner and Cynthia Brown (East Carolina University, Greenville, NC); Sherron Jackson and Lisa Kuisel (Medical University of South Carolina, Charleston, SC); Ramamoorthy Nagasubramanian (Nemours Children's Clinic Orlando, Orlando, FL); Cynthia Gauger (Nemours Children's Clinic, Jacksonville, FL); Brian Berman and Mary DeBarr (Rainbow Babies and Children's Hospital, Cleveland, OH); Sharon Singh and Antonella Farrell (Schneider Children's Hospital, New Hyde Park, NY); Banu Aygun and Eileen Hansbury (St. Jude Children's Research Hospital, Memphis, TN); Scott Miller and Kathy Rey (SUNY Downstate, Brooklyn, NY); Isaac Odame, Nagina Parmar, and Manuella Merelles-Pulcinni (The Hospital for Sick Children, Toronto ON Canada); Zora R Rogers and Leah Adix (The University of Texas Southwestern Medical Center, Dallas, TX); Lee Hilliard and Jeanine Dumas (University of Alabama, Birmingham, AL); Michelle Neier and Stephanie Farias (University of Medicine and Dentistry of New Jersey, New Brunswick, NJ); Ofelia Alvarez and Patrice Williams (University of Miami, Miami, FL); Rathi Iyer and Mary T. Walker (University of Mississippi Medical Center, Jackson, MS); Hamayun Imran and Stephanie Durggin (University of South Alabama, Mobile, AL); Elizabeth Yang (Vanderbilt University, Nashville, TN); Sharada Sarnaik and Mary Murphy (Wayne State University, Detroit, MI).