Portal de Periódicos da CAPES

Heparin-like polymers derived from dextran enhance colonic anastomosis resistance to leakage

1996; Wiley; Volume: 31; Issue: 3 Linguagem: Inglês

10.1002/(sici)1097-4636(199607)31

1097-4636

Anne Meddahi, Jacques Benoit, N.Y. Ayoub, Alain S�zeur, Denis Barritault,

Esophageal Cancer Research and Treatment

A new tissue repair agent, RGTA11, is described for its ability to enhance colonic anastomosis repair and resistance to leakage. RGTA11 is a dextran derivative containing 110% carboxymethyl groups, 2.6% carboxymethyl benzylamide groups, and 36.6% carboxymethyl benzylamide sulfonate groups. RGTA11 was deemed efficient to protect the heparin-binding growth factors FGF2 against trypsin digestion. By this property RGTA11 mimicked heparin or heparan sulfate. We have also found that RGTA11 protected TGFβ1 against trypsin digestion while heparin did not. RGTA11 was then tested in an in vivo wound-healing model of colonic anastomosis. Our results indicate that after 48 h, RGTA11- or RGTA11/FGF-2-treated animals presented a resistance of the anastomosis to leakage which was increased twofold (p < 0.05) over untreated controls. After 96 h and until day 7 there was no more difference with control animals. Our results suggest that RGTA11 presents potential clinical interest by preventing earlier leakage of colonic anastomosis. © 1996 John Wiley & Sons, Inc.