Portal de Periódicos da CAPES

An automated system using spatial oversampling for optical mapping in murine atria. Development and validation with monophasic and transmembrane action potentials

2014; Elsevier BV; Volume: 115; Issue: 2-3 Linguagem: Inglês

10.1016/j.pbiomolbio.2014.07.012

1873-1732

Ting Yu, Fahima Syeda, Andrew P. Holmes, Benjamin Osborne, Hamid Dehghani, Keith L. Brain, Paulus Kirchhof, Larissa Fabritz,

Force Microscopy Techniques and Applications

We developed and validated a new optical mapping system for quantification of electrical activation and repolarisation in murine atria. The system makes use of a novel 2nd generation complementary metal-oxide-semiconductor (CMOS) camera with deliberate oversampling to allow both assessment of electrical activation with high spatial and temporal resolution (128 × 2048 pixels) and reliable assessment of atrial murine repolarisation using post-processing of signals. Optical recordings were taken from isolated, superfused and electrically stimulated murine left atria. The system reliably describes activation sequences, identifies areas of functional block, and allows quantification of conduction velocities and vectors. Furthermore, the system records murine atrial action potentials with comparable duration to both monophasic and transmembrane action potentials in murine atria.