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Determination of Sucralose in Splenda and a Sugar-Free Beverage Using High-Performance Anion-Exchange Chromatography with Pulsed Amperometric Detection

2004; American Chemical Society; Volume: 52; Issue: 14 Linguagem: Inglês

10.1021/jf049974r

1520-5118

Valoran P. Hanko, Jeffrey S. Rohrer,

Meat and Animal Product Quality

Sucralose is a chlorinated carbohydrate nonnutritive sweetener of food and beverage products. The determination of sucralose in food and beverages is important to ensure consistency in product quality. Sucralose was determined in two commercial products without sample preparation using high-performance anion-exchange (HPAE) chromatography coupled with pulsed amperometric detection (PAD). Sucralose was determined with a 10 min isocratic separation. To determine sucralose and other carbohydrates (e.g., dextrose) simultaneously, a gradient separation was developed. The linear range of electrochemical response extended over 3 orders of magnitude, from 0.01 (LOD) to 40 μM (16 μg/mL; 25 μL injection). High precision, high spike recovery, and method ruggedness were observed for both samples. Keywords: Sucralose; anion-exchange chromatography; HPAE-PAD; pulsed amperometric detection; integrated pulsed amperometric detection; HPLC; carbohydrates; artificial sweeteners; Diet Rite; Splenda