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1D Saturation Transfer Difference NMR Experiments on Living Cells: The DC‐SIGN/Oligomannose Interaction

2004; Wiley; Volume: 44; Issue: 2 Linguagem: Inglês

10.1002/anie.200461574

1521-3773

Silvia Mari, Diego Serrano‐Gómez, F. Javier Cañada, Ángel L. Corbí, Jesús Jiménez‐Barbero,

Monoclonal and Polyclonal Antibodies Research

SIGN of the times: Receptor–ligand interactions in which integral membrane proteins are involved can be detected directly by 1D saturation transfer difference (STD) NMR experiments on living cells (see 1H NMR (a) and STD (b) spectra of mannan with K562-CD209 cells). The technique can be used for molecular recognition in drug-screening processes and is demonstrated for the DC-SIGN receptor.