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Giemsa banding of meiotic chromosomes with description of a procedure for cytological preparations from solid tissues

1972; Karger Publishers; Volume: 11; Issue: 6 Linguagem: Inglês

10.1159/000130219

1424-8581

A.D. Stock, D.B. Burnham, Tsai‐Ching Hsu,

DNA Repair Mechanisms

A procedure is described for preparing solid tissues for Giemsa banding and other cytological applications. Carnoy-fixed tissues are transferred to 60 % acetic acid to dissociate the cells, which are then pipetted onto prewarmed slides and allowed to air dry. Preliminary results obtained with this procedure and demonstrating G-banding of meiotic chromosomes prepared from mouse testicular tissue are presented.