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Histological assessment of cochlear damage in basilar membrane experiments

1980; Acoustical Society of America; Volume: 68; Issue: S1 Linguagem: Inglês

10.1121/1.2004729

1520-9024

D. G. B. Leonard, Shyam M. Khanna,

Noise Effects and Management

The condition of the cochleas in the basilar membrane experiments [Khanna and Leonard, J. Acoust. Soc. Am. Suppl. 1 67, S46 (1980)] was assessed by gross potential responses (CM and NI) and by histological assessment. For histology, cochleas were fixed in vivo immediately after the experiments. An epon-embedded surface preparation technique was used, as described by Liberman and Beil [Acta Otolaryngol. (1978)]. In an early series of cochleas, surgical preparation for the experiments included drilling of the petrous bone and nonsterile techniques. In the second series of experiments, no drilling was used and precautions were taken to minimize introduction of bacteria into the cochlea. Some of the factors which seem to be related to the damage were: (a) condition of the hair cells and stereocilia; (b) presence of macrophages; and (c) presence of clear vesicles under the inner hair cells. An attempt is made to correlate the histological damage with the degree of damage as measured with electrical potentials.