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PARAFAC2 and MCR-ALS quantification of Diltiazem antihypertensor based on a kinetic spectrophotometric methodology

2007; Elsevier BV; Volume: 89; Issue: 2 Linguagem: Inglês

10.1016/j.chemolab.2007.06.003

1873-3239

João M.M. Leitão, Joaquim C. G. Esteves da Silva,

Pesticide Residue Analysis and Safety

A Diltiazem kinetic spectrophotometric UV–Vis method, based on a reaction of the Diltiazem with hidroxylamine and a ferric salt, was used for the quantification of Diltiazem in different pharmaceutical formulations. This method is based on the acquisition of three-way data structures [wavelength (nm) × time (s) × concentration (mg/L)] followed by chemometric analysis by an appropriate PARAFAC2 or MCR-ALS second-order calibration model. The results obtained are compared with those obtained by direct determination, at maximum wavelength, and by the United States Pharmacopeia (USP) standard chromatographic method. For all the pharmaceutical formulations analysed good quantification results were found with PARAFAC2 and MCR-ALS second-order calibration models. For bulk drug analysis, detection limits of 6 and 2 mg/L, and for pharmaceutical formulations analysis, an average detection limit of 41 and 39 mg/L were found, respectively with PARAFAC2 and MCR-ALS.