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Synthetic Ligand-Coated Magnetic Nanoparticles for Microfluidic Bacterial Separation from Blood

2013; American Chemical Society; Volume: 14; Issue: 1 Linguagem: Inglês

10.1021/nl3047305

1530-6992

Jung‐Jae Lee, Kyung Jae Jeong, Michinao Hashimoto, Albert Kwon, Alina Y. Rwei, Sahadev Shankarappa, Jonathan H. Tsui, Daniel S. Kohane,

Biosensors and Analytical Detection

Bacterial sepsis is a serious clinical condition that can lead to multiple organ dysfunction and death despite timely treatment with antibiotics and fluid resuscitation. We have developed an approach to clearing bacteria and endotoxin from the bloodstream, using magnetic nanoparticles (MNPs) modified with bis-Zn-DPA, a synthetic ligand that binds to both Gram-positive and Gram-negative bacteria. Magnetic microfluidic devices were used to remove MNPs bound to Escherichia coli, a Gram-negative bacterium commonly implicated in bacterial sepsis, from bovine whole blood at flows as high as 60 mL/h, resulting in almost 100% clearance. Such devices could be adapted to clear bacteria from septicemic patients.