Cytolytic effect of human anti‐Gal IgM and complement on porcine endothelial cells: A kinetic analysis
1994; Wiley; Volume: 1; Issue: 2 Linguagem: Inglês
10.1111/j.1399-3089.1994.tb00058.x
ISSN1399-3089
AutoresL Borche, Karen Thibaudeau, Jean‐Marc Navenot, Jean‐Paul Soulillou, Dominique Blanchard,
Tópico(s)Organ Transplantation Techniques and Outcomes
ResumoAbstract: The binding of human natural antibodies to porcine endothelial cells is the first step leading to activation of complement and lysis of the cells. The kinetic of incorporation of propidium iodide into porcine endothelial cell line SVPAEC/6A or peripheral blood lymphocytes was investigated to monitor the permeabilization of target cells by membrane attack complex. In less than 5 min, more than 90% of porcine cells were highly fluorescent upon incubation with human sera, showing they incorporate propidium iodide. The lysis occurred neither with adult sera pretreated with dithiothreitol nor with umbilical cord sera, suggesting that lytic antibodies belong to the IgM class. By using antibodies purified from human sera, the bulk of lytic activity was found to be associated with IgM specific for the Galal‐3Gal epitope, whereas IgG were not able to lyse the porcine cells. These antibodies could be readily absorbed on rabbit red blood cells known to express the main epitope target of xeno human antibodies. Finally, our results show that permeabilization of porcine endothelial cells is a very early phenomenon, probably directly associated with the biological process responsible for the hyperacute rejection.
Referência(s)