Characterization In Vitro and In Vivo of a Pandemic H1N1 Influenza Virus from a Fatal Case
2013; Public Library of Science; Volume: 8; Issue: 1 Linguagem: Inglês
10.1371/journal.pone.0053515
ISSN1932-6203
AutoresAriel Rodríguez-Frandsen, Ana Falcón, María Teresa Cuevas, Francisco Pozo, Susana Guerra, Blanca Garcı́a-Barreno, Pamela Martínez-Orellana, Pilar Pérez‐Breña, María Montoya, José A. Melero, Manuel Pizarro, Juan Ortı́n, Inmaculada Casas, Amelia Nieto,
Tópico(s)Immune Response and Inflammation
ResumoPandemic 2009 H1N1 (pH1N1) influenza viruses caused mild symptoms in most infected patients. However, a greater rate of severe disease was observed in healthy young adults and children without co-morbid conditions. Here we tested whether influenza strains displaying differential virulence could be present among circulating pH1N1 viruses. The biological properties and the genotype of viruses isolated from a patient showing mild disease (M) or from a fatal case (F), both without known co-morbid conditions were compared in vitro and in vivo. The F virus presented faster growth kinetics and stronger induction of cytokines than M virus in human alveolar lung epithelial cells. In the murine model in vivo, the F virus showed a stronger morbidity and mortality than M virus. Remarkably, a higher proportion of mice presenting infectious virus in the hearts, was found in F virus-infected animals. Altogether, the data indicate that strains of pH1N1 virus with enhanced pathogenicity circulated during the 2009 pandemic. In addition, examination of chemokine receptor 5 (CCR5) genotype, recently reported as involved in severe influenza virus disease, revealed that the F virus-infected patient was homozygous for the deleted form of CCR5 receptor (CCR5Δ32).
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