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Sperm penetration and transformation of sperm entry site in eggs of the freshwater teleostRhodeus ocellatus ocellatus

1996; Wiley; Volume: 229; Issue: 2 Linguagem: Inglês

10.1002/(sici)1097-4687(199608)229

1097-4687

Tadayuki Ohta, Chie Nashirozawa,

Reproductive Biology and Fertility

Journal of MorphologyVolume 229, Issue 2 p. 191-200 Sperm penetration and transformation of sperm entry site in eggs of the freshwater teleost Rhodeus ocellatus ocellatus Tadayuki Ohta, Corresponding Author Tadayuki Ohta Department of Biological Science, Aichi University of Education, Kariya City 448, JapanDepartment of Biological Science, Aichi University of Education, Kariya City 448, JapanSearch for more papers by this authorChie Nashirozawa, Chie Nashirozawa Department of Biological Science, Aichi University of Education, Kariya City 448, JapanSearch for more papers by this author Tadayuki Ohta, Corresponding Author Tadayuki Ohta Department of Biological Science, Aichi University of Education, Kariya City 448, JapanDepartment of Biological Science, Aichi University of Education, Kariya City 448, JapanSearch for more papers by this authorChie Nashirozawa, Chie Nashirozawa Department of Biological Science, Aichi University of Education, Kariya City 448, JapanSearch for more papers by this author First published: August 1996 https://doi.org/10.1002/(SICI)1097-4687(199608)229:2 3.0.CO;2-4Citations: 5AboutPDF ToolsRequest permissionExport citationAdd to favoritesTrack citation ShareShare Give accessShare full text accessShare full-text accessPlease review our Terms and Conditions of Use and check box below to share full-text version of article.I have read and accept the Wiley Online Library Terms and Conditions of UseShareable LinkUse the link below to share a full-text version of this article with your friends and colleagues. Learn more.Copy URL Share a linkShare onFacebookTwitterLinked InRedditWechat Abstract Eggs of bony fishes are enveloped by an egg envelope (chorion) in which a micropyle is present near the animal pole. Therefore, sperm penetration into the eggs is limited to the sperm entry site (SES), a region of plasma membrane just beneath the micropyle. In rose bitterling eggs, the SES transforms from a tuft of microvilli into a swollen mass (SM) that continues to plug the micropyle after sperm penetration. The present observations using the rose bitterling Rhodeus ocellatus ocellatus were conducted to examine: 1) whether or not sperm penetration is necessary for formation of the SM and 2) whether or not actin microfilaments are involved in the formation of the SM. Water activation without sperm transformed the SES from a tuft of microvilli into the SM, although it took a longer time for the transformation and the SMs were smaller than in the case of inseminated eggs. The SES presumably has the ability to transform into the SM upon activation of eggs in the present species. Cytochalasin B, which acts on actin microfilaments, did not prevent formation of the SM, irrespective of insemination or activation. The present observations suggest that sperm penetration is not necessary for SM formation and actin microfilaments do not participate in SM formation. © 1996 Wiley-Liss, Inc. Citing Literature Volume229, Issue2August 1996Pages 191-200 RelatedInformation