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PCR detection of Listeria monocytogenes in ‘gravad’ rainbow trout

1997; Elsevier BV; Volume: 35; Issue: 3 Linguagem: Inglês

10.1016/s0168-1605(97)01246-4

1879-3460

Hans Ericsson, Per Stålhandske,

Essential Oils and Antimicrobial Activity

'Gravad' rainbow trout artificially contaminated with Listeria monocytogenes was analyzed by use of a 4 h enrichment period followed by extraction of DNA and PCR amplification. This procedure made it possible to detect 10-100 cfu L. monocytogenes per gram 'gravad' rainbow trout, within 12 h. After a prolonged enrichment period of 24 h, numbers as low as 1-10 cfu L. monocytogenes per gram could be detected. The method described may be a useful tool for screening samples of 'gravad' rainbow trout for the presence of L. monocytogenes, since it is sensitive, rapid and simple.