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Formation of intermediates in the replication of phage lambda DNA

1967; Elsevier BV; Volume: 28; Issue: 1 Linguagem: Inglês

10.1016/s0022-2836(67)80077-9

1089-8638

Lois Ann Salzman, Arthur Weissbach,

Advanced biosensing and bioanalysis techniques

After induction of Escherichia coli K12(λ) by ultraviolet light, a large part of the newly synthesized DNA is present as intermediate forms which are clearly distinguishable from mature λ DNA. One of the new DNA species is very sensitive to shearing and shows a relatively high sedimentation rate when compared to normal λ DNA. Its properties are consistent with those of a long, linear, concatenated structure. The intermediate forms of λ DNA are apparently formed from smaller DNA molecules the size of mature λ DNA. In the latter part of the latent period just before cell lysis, these large intermediates are broken down to DNA of the size found in the normal phage particle. λsusA and λsusD mutants have been found to accumulate large amounts of the intermediate during λ DNA synthesis and are unable to convert them to mature phage DNA.