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The DNA hybridization assay using single-walled carbon nanotubes as ultrasensitive, long-term optical labels

2006; IOP Publishing; Volume: 17; Issue: 14 Linguagem: Inglês

10.1088/0957-4484/17/14/016

1361-6528

Eung‐Soo Hwang, Chengfan Cao, Sanghyun Hong, Hye-Jin Jung, Chang-Yong Cha, Jae-Boong Choi, Young Jin Kim, Seunghyun Baik,

Nanopore and Nanochannel Transport Studies

Single walled carbon nanotubes (SWNTs) exhibit strong Raman signals as well as fluorescence emissions in the near infrared region. Such signals do not blink or photobleach under prolonged excitation, which is an advantage in optical nano-biomarker applications. In this paper, we present single-stranded DNA conjugated SWNT probes to locate a particular sequence of DNA within a complex genome. Chromosomal DNAs of human fibroblasts and Escherichia coli are used as a target and a control, respectively. Southern blotting, which uses photostable Raman signals of nanotubes instead of fluorescent dyes, demonstrates excellent sensitivity and specificity of the probes. The results show that SWNTs may be used as generic nano-biomarkers for the precise detection of specific kinds of genes.