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THE INFLUENCE OF VARIOUS CULTURAL FACTORS ON ANTHER CULTURE OF FOUR CULTIVARS OF SPRING WHEAT ( Triticum aestivum L.)

1990; Canadian Science Publishing; Volume: 70; Issue: 1 Linguagem: Inglês

10.4141/cjps90-020

1918-1833

Linda McGregor, Alan McHughen,

Plant Genetic and Mutation Studies

Four spring wheat cultivars (Katepwa, Columbus, Chris and Israel 676) were compared for androgenic ability. Temperature, medium, agar concentration and cold pretreatment were examined to find the optimal combinations for anther culture of Katepwa and Columbus in particular. At an incubation temperature of 30 °C, anthers of Chris were significantly more responsive than the other genotypes. In three temperature treatments (26, 28 and 30 °C) the potato extract medium (P1) was superior to the synthetic medium (N6) (Chu 1978) for callus induction and green plant regeneration. Over all genotypes, liquid media provided greater anther response than either 0.8% or 1.8% agar. At 28 °C, cultured anthers showed no significant increase in callus formation after being subjected to a cold pretreatment. A cold pretreatment prior to culture at 30 °C elicited a significant decrease in callus formation as compared with no cold pretreatment. However, maximum callus induction and regeneration frequencies for Katepwa and Columbus were obtained at 28 °C in the P1 liquid medium with cold pretreatment. An incubation temperature of 26 °C was inferior to 28° and 30 °C for callus induction and regeneration.Key words: Anther culture, wheat, genotype, incubation temperature, media, agar concentration