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Regulation of Junonia coenia densovirus P9 promoter expression

2007; Wiley; Volume: 16; Issue: 5 Linguagem: Inglês

10.1111/j.1365-2583.2007.00759.x

1365-2583

Paul D. Shirk, Hervé Bossin, Richard B. Furlong, J. L. Gillett,

CRISPR and Genetic Engineering

Abstract Transcriptional activity of the Junonia coenia densovirus ( Jc DNV) P9 promoter depends on a 557‐bp sequence located within the overlapping 3′ sequences for viral capsid and nonstructural genes. Utilizing a somatic transformation assay to assess Jc DNV promoter activity in Drosophila melanogaster and Plodia interpunctella , viral sequences were subjected to deletional analysis. Removal of a 685‐bp fragment reduced P9‐driven expression to background levels. Inclusion of a second expression cassette demonstrated vector persistence and confirmed somatic transformation. P9 promoter‐driven expression was restored by insertion of a 557‐bp Jc DNV fragment or by inclusion of a heterologous baculovirus hr5 enhancer. Consensus polycomb transcriptional factor binding sites were identified within the 557‐bp fragment, which suggests a potential role in regulating densoviral transcription.