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On the Chemistry of Ingenol, IV [1]

1991; De Gruyter; Volume: 46; Issue: 10 Linguagem: Inglês

10.1515/znb-1991-1022

1865-7117

Ratnam Bagavathi, B. Sorg, E. Hecker,

Plant-based Medicinal Research

A simple and fast procedure was worked out to prepare from the commercially available seeds of Euphorbia lathyris L. substantial amounts of the polyfunctional tetracyclic diterpene ingenol (la). Ingenol-3,4:5,20-diacetonide (1d) was treated with five different bases at two different temperatures to achieve epimerization to 8-epiingenol. In any case unchanged 1 d was obtained. By reduction of ingenol-3,4: 5,20-diacetonide (1d) with sodium in ether/2-propanol (9 R)-9-deoxo-9-hydroxyingenol-3,4:5,20-diacetonide (2b) was obtained and converted to the corresponding 5,20-monoacetonide 2e. Reduction of ingenol-5,20-acetonide (le) with LiAlH 4 in tetrahydrofuran furnished the epimeric (9 S)-9-deoxo-9-hydroxyingenol-5,20-acetonide (3c).The (9 R)-monoacetonide 2e was used to prepare the 3-tetradecanoate 2c of (9 R)-9-deoxo-9-hydroxyingenol (2a). From the (9 S)-monoacetonide 3 c four 3-esters [butyrate (3d), tetradecanoate (3e), eicosanoate (3f), benzoate (3g)] of (9S)-9-deoxo-9-hydroxyingenol (3 a) were synthesized. The 3,4 : 5,20-diacetonides of 2a and 3a, i.e. 2b/3b were used to provide the 9-hexadecanoates 2 f and 3j. The irritant activities of the 3-esters of the (9 S)-series depend strongly on the nature of the acyl group, thus furnishing, e. g. the moderately irritant tetradecanoate 3e [compared to corresponding 3-esters lb of ingenol (la)] as well as the highly active benzoate 3g of 3 a. The (9 R)-3-tetradecanoate 2c exhibits comparable activity as its (9 S)-analogue 3e. Thus, the spatial orientation (R or S) of the 9-hydroxy function plays a significant but not a crucial role for irritant activity. Both epimeric (9 R)/(9 S)-hexadecanoates 2f/3j were non-irritant.