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Chromosomal location of AFLP markers in common wheat utilizing nulli-tetrasomic stocks

2000; NRC Research Press; Volume: 43; Issue: 2 Linguagem: Inglês

10.1139/gen-43-2-298

1480-3321

Xiu-Qiang Huang, Friedrich J. Zeller, S. L. K. Hsam, Gerhard Wenzel, Volker Mohler,

Genetic Mapping and Diversity in Plants and Animals

Amplified fragment length polymorphism (AFLP) markers with a total of 256 EcoRI + ANN - MseI + CNN primer combinations were investigated employing the common wheat cultivar Triticum aestivum 'Chinese Spring.' On average, 103 fragments per primer combination were amplified, ranging from a maximum of 226 fragments to a minimum of 18 fragments. The primer combinations E + AAA - M + CNN and E + ATT - M + CNN produced very few distinct fragments. By using 15 randomly chosen EcoRI + ANN - MseI + CNN primer combinations, 928 AFLP markers were allocated to wheat chromosomes, of which 131 were assigned to specific chromosome arms. These AFLP markers were locus-specific and randomly distributed on the different chromosomes. In addition, 6 and 41 AFLP markers were simultaneously absent in two nulli-tetrasomics (NTs) of both homoeologous and non-homoeologous groups, respectively, whereas additional fragments were detected in N1BT1A, N5AT5D, and N6BT6A lines.