Increased expression of epidermal fatty acid binding protein, cofilin, and 14-3-3-σ (stratifin) detected by two-dimensional gel electrophoresis, mass spectrometry and microsequencing of drug-resistant human adenocarcinoma of the pancreas
1999; Wiley; Volume: 20; Issue: 14 Linguagem: Inglês
10.1002/(sici)1522-2683(19991001)20
ISSN1522-2683
AutoresPranav Sinha, Gero Hütter, Eckart Köttgen, Manfred Dietel, Dirk Schadendorf, Hermann Lage,
Tópico(s)Metabolomics and Mass Spectrometry Studies
ResumoELECTROPHORESISVolume 20, Issue 14 p. 2952-2960 Research Article Increased expression of epidermal fatty acid binding protein, cofilin, and 14-3-3-σ (stratifin) detected by two-dimensional gel electrophoresis, mass spectrometry and microsequencing of drug-resistant human adenocarcinoma of the pancreas Pranav Sinha, Corresponding Author Pranav Sinha [email protected] Institut für Laboratoriumsmedizin und PathobiochemieInstitut für Laboratoriumsmedizin und Pathobiochemie, Universitätsklinikum Charité, Medizinische Fakultät der Humboldt Universität zu Berlin, Schumannstraße 20−21, Berlin, Germany, Fax: +49-30-2802-8422===Search for more papers by this authorGero Hütter, Gero Hütter Institut für Laboratoriumsmedizin und PathobiochemieSearch for more papers by this authorEckart Köttgen, Eckart Köttgen Institut für Laboratoriumsmedizin und PathobiochemieSearch for more papers by this authorManfred Dietel, Manfred Dietel Institut für Pathologie, Universitätsklinikum Charité, Berlin, GermanySearch for more papers by this authorDirk Schadendorf, Dirk Schadendorf Klinische Kooperationseinheit für Dermatoonkologie (DKFZ) an der Hautklinik Mannheim, GermanySearch for more papers by this authorHermann Lage, Hermann Lage Institut für Pathologie, Universitätsklinikum Charité, Berlin, GermanySearch for more papers by this author Pranav Sinha, Corresponding Author Pranav Sinha [email protected] Institut für Laboratoriumsmedizin und PathobiochemieInstitut für Laboratoriumsmedizin und Pathobiochemie, Universitätsklinikum Charité, Medizinische Fakultät der Humboldt Universität zu Berlin, Schumannstraße 20−21, Berlin, Germany, Fax: +49-30-2802-8422===Search for more papers by this authorGero Hütter, Gero Hütter Institut für Laboratoriumsmedizin und PathobiochemieSearch for more papers by this authorEckart Köttgen, Eckart Köttgen Institut für Laboratoriumsmedizin und PathobiochemieSearch for more papers by this authorManfred Dietel, Manfred Dietel Institut für Pathologie, Universitätsklinikum Charité, Berlin, GermanySearch for more papers by this authorDirk Schadendorf, Dirk Schadendorf Klinische Kooperationseinheit für Dermatoonkologie (DKFZ) an der Hautklinik Mannheim, GermanySearch for more papers by this authorHermann Lage, Hermann Lage Institut für Pathologie, Universitätsklinikum Charité, Berlin, GermanySearch for more papers by this author First published: 25 October 1999 https://doi.org/10.1002/(SICI)1522-2683(19991001)20:14 3.0.CO;2-HCitations: 110AboutPDF ToolsRequest permissionExport citationAdd to favoritesTrack citation ShareShare Give accessShare full text accessShare full-text accessPlease review our Terms and Conditions of Use and check box below to share full-text version of article.I have read and accept the Wiley Online Library Terms and Conditions of UseShareable LinkUse the link below to share a full-text version of this article with your friends and colleagues. Learn more.Copy URL Abstract In order to study possible mechanisms leading to chemoresistance in pancreatic adenocarcinoma we examined the global protein expression of pancreatic cancer cells in vitro. We used a cell culture model derived from the adenocarcinoma of the pancreas (EPP85-181P). A classical multidrug-resistant subline, EPP85-181RDB, selected in presence of daunorubicin, and an atypical multidrug-resistant cell variant, EPP85-181RNOV, selected in presence of mitoxantrone, were analyzed using two-dimensional electrophoresis. After staining and image analysis, spots of interest were isolated using preparative two-dimensional electrophoresis and subjected to mass spectrometry and microsequencing. Three proteins, E-FABP, cofilin, and 14-3-3-σ (stratifin), were overexpressed in chemoresistant cell lines. Cofilin was present in both multidrug in chemoresistant cell lines. Cofilin was present in both multidrug-resistant cell lines. 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