Effect of nitrogen-starvation, light intensity and iron on triacylglyceride/carbohydrate production and fatty acid profile of Neochloris oleoabundans HK-129 by a two-stage process
2014; Elsevier BV; Volume: 155; Linguagem: Inglês
10.1016/j.biortech.2013.12.109
ISSN1873-2976
AutoresXian Sun, Yu Cao, Hui Xu, Yan Liu, Jianrui Sun, Dairong Qiao, Yi Cao,
Tópico(s)Enzyme Catalysis and Immobilization
ResumoTriacylglyceride (TAG) and carbohydrate are potential feedstock for biofuels production. In this study, a two-stage process was applied for enhancing TAG/carbohydrate production in the selected microalgae – Neochloris oleoabundans HK-129. In stage I, effects of nitrogen, light intensity and iron on cell growth were investigated, and the highest biomass productivity of 292.83 ± 5.83 mg/L/d was achieved. In stage II, different nitrogen-starvation periods, light intensities and iron concentrations were employed to trigger accumulation of TAG and carbohydrate. The culture under 2-day N-starvation, 200 μmol/m2/s light intensity and 0.037 mM Fe3+ concentration produced the maximum TAG and carbohydrate productivity of 51.58 mg/L/d and 90.70 mg/L/d, respectively. Nitrogen starvation period and light intensity had marked effects on TAG/carbohydrate accumulation and fatty acids profile, compared to iron concentration. The microalgal lipid was mainly composed of C16/C18 fatty acids (90.02%), saturated fatty acids (29.82%), and monounsaturated fatty acids (32.67%), which is suitable for biodiesel synthesis.
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