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A synthetic luciferin improves bioluminescence imaging in live mice

2014; Nature Portfolio; Volume: 11; Issue: 4 Linguagem: Inglês

10.1038/nmeth.2839

1548-7105

Melanie S. Evans, Joanna Chaurette, Spencer T. Adams, G. Randheer Reddy, Miranda A. Paley, Neil Aronin, Jennifer A. Prescher, Stephen C. Miller,

Cell Image Analysis Techniques

The synthetic firefly luciferase substrate CycLuc1 offers brighter bioluminescence and improved imaging in mouse models at lower doses than the standard D-luciferin. Firefly luciferase is the most widely used optical reporter for noninvasive bioluminescence imaging (BLI) in rodents. BLI relies on the ability of the injected luciferase substrate D-luciferin to access luciferase-expressing cells and tissues within the animal. Here we show that injection of mice with a synthetic luciferin, CycLuc1, improves BLI with existing luciferase reporters and enables imaging in the brain that could not be achieved with D-luciferin.