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Matrix metalloproteinases in type 2 diabetes and non-diabetic controls: effects of short-term and chronic hyperglycaemia

2011; Termedia Publishing House; Volume: 2; Linguagem: Inglês

10.5114/aoms.2011.22081

1896-9151

Krzysztof Lewandowski, Ewa Banach, Małgorzata Bieńkiewicz, Andrzej Lewiński,

Blood Coagulation and Thrombosis Mechanisms

Introduction: The role of matrix metalloproteinases (MMPs) in type 2 diabetes mellitus (DM) is not clear as increased activation of MMPs in the vasculature contrasts with decreased activity of MMPs in the kidneys, contributing to development of nephropathy.Material and methods: We measured serum MMP-2 and MMP-9 in 22 subjects with type 2 DM age (mean ± SD) 56.7 ±16.8 years, BMI 31.8 ±4.6 kg/m 2 , HbA 1c 8.45 ±1.78% and in 32 controls, age 39.2 ±16.0 years, BMI 35.2 ±8.5 kg/m 2 .In 15 subjects with 2 DM we also measured MMP-2 and MMP-9 at discharge from hospital and after 3 months (n = 8).In controls, MMP-2 and -9 were also measured during 75 g oral glucose tolerance test (OGTT).Results: Concentrations of MMP-2 and MMP-9 were lower in subjects with type 2 DM (219 ±62 ng/ml vs. 305 ±63 ng/ml and 716 ±469 ng/ml vs. 1285 ±470 ng/ml, for MMP-2 and MMP-9, respectively, p < 0.05).MMP-9 concentrations fell at 120 min of OGTT from 1675 ±372 ng/ml to 1276 ±422 ng/ml (p < 0.05).In diabetic subjects there was a correlation between MMP-9 and HbA 1c (r = 0.51, p < 0.05).In subjects with diabetes there was a fall of HbA 1c from 9.77 ±1.76% to 8.36 ±1.54% (p < 0.01), at three months post-discharge.There was no difference in MMP-2, but there was a fall in MMP-9 at three months post-discharge in comparison to concentrations observed at admission (854 ±560 ng/ml vs. 500 ±235 ng/ml, p = 0.02).Conclusions: Matrix metalloproteinases in type 2 and MMP-9 concentrations were lower in subjects with 2 DM than in non-diabetic controls.Regulation of MMPs appears to be complex as hyperglycaemia during OGTT results in a decrease in MMP-9, while chronic hyperglycaemia, reflected by HbA 1c , correlates with MMP-9 concentrations in subjects with 2 DM.