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Sphingosine-1-phosphate effects on PKC isoform expression in human osteoblastic cells

2001; Elsevier BV; Volume: 65; Issue: 3 Linguagem: Inglês

10.1054/plef.2001.0302

1532-2823

Judith Lampasso, Angela R. Kamer, Joseph E. Margarone, Rosemary Dziak,

Protein Kinase Regulation and GTPase Signaling

Abstract Sphingosine-1-phosphate (S1P) has been shown to participate in the proliferative process in human osteoblasts. 1 The mitogenic effect of S1P has been postulated to involve two signaling pathways, the Gi linked protein receptor pathway and the PKC pathway. To define the possible role of PKC isoforms in osteoblastic cell proliferation, the effects of S1P on PKC isoform expression was determined. While PKC λ was minimally detected, the isoforms α , δ and ι were all found to be highly expressed by the human osteoblast. In human osteoblastic cells, S1P induced a 25% increase in the expression of PKC α and approximately a 30% increase in the expression of PKC ι . S1P did not have an effect on PKC δ expression. Pretreatment with pertussis toxin (PT) led to an inhibition of the observed S1P effects on the expression of the α and ι isoforms.