AtSPO11-1 is necessary for efficient meiotic recombination in plants
2001; Springer Nature; Volume: 20; Issue: 3 Linguagem: Inglês
10.1093/emboj/20.3.589
ISSN1460-2075
Autores Tópico(s)Microtubule and mitosis dynamics
ResumoArticle1 February 2001free access AtSPO11-1 is necessary for efficient meiotic recombination in plants Mathilde Grelon Corresponding Author Mathilde Grelon Station de Génétique et d'Amélioration des Plantes, INRA de Versailles, Route de St-Cyr, 78026 Versailles, Cedex, France Search for more papers by this author Daniel Vezon Daniel Vezon Station de Génétique et d'Amélioration des Plantes, INRA de Versailles, Route de St-Cyr, 78026 Versailles, Cedex, France Search for more papers by this author Ghislaine Gendrot Ghislaine Gendrot Station de Génétique et d'Amélioration des Plantes, INRA de Versailles, Route de St-Cyr, 78026 Versailles, Cedex, France Search for more papers by this author Georges Pelletier Georges Pelletier Station de Génétique et d'Amélioration des Plantes, INRA de Versailles, Route de St-Cyr, 78026 Versailles, Cedex, France Search for more papers by this author Mathilde Grelon Corresponding Author Mathilde Grelon Station de Génétique et d'Amélioration des Plantes, INRA de Versailles, Route de St-Cyr, 78026 Versailles, Cedex, France Search for more papers by this author Daniel Vezon Daniel Vezon Station de Génétique et d'Amélioration des Plantes, INRA de Versailles, Route de St-Cyr, 78026 Versailles, Cedex, France Search for more papers by this author Ghislaine Gendrot Ghislaine Gendrot Station de Génétique et d'Amélioration des Plantes, INRA de Versailles, Route de St-Cyr, 78026 Versailles, Cedex, France Search for more papers by this author Georges Pelletier Georges Pelletier Station de Génétique et d'Amélioration des Plantes, INRA de Versailles, Route de St-Cyr, 78026 Versailles, Cedex, France Search for more papers by this author Author Information Mathilde Grelon 1, Daniel Vezon1, Ghislaine Gendrot1 and Georges Pelletier1 1Station de Génétique et d'Amélioration des Plantes, INRA de Versailles, Route de St-Cyr, 78026 Versailles, Cedex, France *Corresponding author. E-mail: [email protected] The EMBO Journal (2001)20:589-600https://doi.org/10.1093/emboj/20.3.589 PDFDownload PDF of article text and main figures. ToolsAdd to favoritesDownload CitationsTrack CitationsPermissions ShareFacebookTwitterLinked InMendeleyWechatReddit Figures & Info The Saccharomyces cerevisiae Spo11 protein catalyses DNA double-strand breaks (DSBs) that initiate meiotic recombination. The model plant Arabidopsis thaliana possesses at least three SPO11 homologues. T-DNA and ethyl-methane sulfonate mutagenesis allowed us to show that meiotic progression is altered in plants in which the AtSPO11-1 gene is disrupted. Both male and female meiocytes formed very few bivalents. Furthermore, no fully synapsed chromosomes were observed during prophase I. Later, in meiosis I, we observed that chromosomes segregated randomly, leading to the production of a large proportion of non-functional gametes. These meiotic aberrations were associated with a drastic reduction in meiotic recombination. Thus, our data show that initiation of meiotic recombination by SPO11- induced DSBs is a mechanism conserved in plants. Furthermore, unlike Drosophila and Caenorhabditis elegans, but like fungi, SPO11 is necessary for normal synapsis in plants. Introduction In contrast to animals, plant organogenesis is a continuous process, and somatic recombination can therefore lead to transmittable genetic variations. Nevertheless, even in plants, meiosis remains the major source of genetic variability. Cross-overs and genic conversions generate recombination between homologous chromosomes, reorganizing the allelic combinations among them. Cross-overs also establish physical links between homologous chromosomes (chiasmata) so that these remain linked together even when the synaptonemal complex (SC) disappears. This mechanism thus ensures proper segregation of chromosomes at anaphase I when chiasmata are released (for a review see Roeder, 1997), allowing random segregation of paternal and maternal chromosomes (interchromosomal recombination). In budding yeast, meiotic recombination is initiated by double-strand breaks (DSBs) (Sun et al., 1989; Cao et al., 1990), which will undergo strand exchange interactions with an intact partner duplex. The formation of DSBs requires at least nine different genes (SPO11, RAD50, MRE11, XRS2, MER2, MEI4, REC102, REC104, REC114) (reviewed in Smith and Nicolas, 1998; Pâques and Haber, 1999). There are now several indications that DSBs in Saccharomyces cerevisiae are initiated by the Spo11 protein, which has been found linked to the 5′ termini of the broken DNA molecules in rad50S yeast mutants (Keeney et al., 1997). Spo11 belongs to a novel family of type II topoisomerase (topoisomerase VI, subunit A), suggesting that Spo11 is the protein that catalyses the formation of DSBs through a 5′-phosphotyrosyl linkage (Bergerat et al., 1997). Sequence comparison of the members of the topoisomerase VI/SPO11 family, as well as structural analyses of one of its members (topoisomerase VI A of Methanococcus jannaschii; Nichols et al., 1999), permitted the definition of several conserved domains involved in DNA binding and cleavage (Bergerat et al., 1997; Aravind et al., 1998; Nichols et al., 1999). Evidence for the generation of DSBs during meiosis of other organisms has only been obtained for Schizosaccharomyces pombe (Cervantes et al., 2000; Zenvirth and Simchen, 2000), but SPO11 homologues have been identified in Drosophila melanogaster (mei-W68; McKim and Hayashi-Hagihara, 1998), Caenorhabditis elegans (Dernburg et al., 1998), S.pombe (REC12; Lin and Smith, 1994), Coprinus cinereus (Cummings et al., 1999; Celerin et al., 2000) and mammals (Keeney et al., 1999; Romanienko and Camerini-Otero, 1999; Shannon et al., 1999; Metzler-Guillemain and de Massy, 2000), suggesting that enzymatic generation of DSBs is conserved among all these organisms. As a matter of fact, meiotic defects (including absence of recombination) were found to be associated with SPO11 disruption not only in yeasts (Klapholz et al., 1985; Atcheson et al., 1987; Giroux et al., 1989; Lin and Smith, 1994), but also in Drosophila (McKim and Hayashi-Hagihara, 1998), C.elegans (Dernburg et al., 1998) and Coprinus (Celerin et al., 2000). Differences between species appeared when authors investigated the relationship between synapsis (homologous chromosome association through the SC) and SPO11 disruption. spo11 mutations in fungi co-ordinately affect recombination and SC formation (Giroux et al., 1989; Celerin et al., 2000), whereas SC formation in Drosophila and C.elegans was found to be independent of SPO11 (Dernburg et al., 1998; McKim et al., 1998). These results underline the importance of studying several independent models to understand the relationship between recombination and synapsis. In addition to DSB formation and synapsis, additional functions in early chromosome pairing (close juxtaposition of homologous chromosomes; Loidl et al., 1994; Weiner and Kleckner, 1994) and S-phase replication rates (Cha et al., 2000) have been assigned to Spo11 in S.cerevisiae. The recent study of Cha et al. (2000) showed that early roles of Spo11 (chromosome pairing and replication control) can be uncoupled from later ones (DSB formation) in mutants where the tyrosine involved in DSB cleavage is replaced by a phenylalanine (spo11Y135F). In plants, very few molecular data have been obtained on meiosis. Even less is known about meiotic recombination: the only functional data come from the analyses on the Arabidopsis dmc1 mutant that was shown to be deficient in bivalent formation during male and female meiosis (Couteau et al., 1999). The Lilium RecA homologues (LIM15 and RAD51) (Terasawa et al., 1995; Anderson et al., 1997) as well as the maize RAD51 protein (Franklin et al., 1999) have been studied by immunolocalization on meiotic chromosomes, implicating these proteins in several aspects of meiosis in plants, including homology searches between chromosomes, pairing and recombination. In addition, several synaptic mutants have been described in Arabidopsis (Ross et al., 1997) that could lead to the characterization of other genes potentially involved in recombination since most of these mutations are thought to reflect defects in recombination (Dawe, 1998). Until now, the only report of Arabidopsis mutants affected in meiotic recombination comes from Masson and Paszkowski (1997), who selected several lines according to their hypersensitivity to X-ray irradiation (Masson et al., 1997). Two of them were found to present defects in somatic and meiotic recombination (Masson and Paszkowski, 1997). Sequencing of the Arabidopsis thaliana genome is nearly complete and allowed Hartung and Puchta (2000) to describe two SPO11 homologues in this model plant. A third SPO11-like coding sequence can be found in the DDBJ/EMBL/GenBank databases (accession No. CAB86036). Until now, SPO11 genes have been found in every eukaryote tested, but only plants possess more than one homologue. The meaning of such sequence redundancy is not yet understood. The study presented here shows that mechanisms by which recombination initiates are conserved between yeast, animals and plants, and that disruption of the AtSPO11-1 gene is sufficient to drastically reduce meiotic recombination and pachytene-stage homologue juxtaposition. Results Isolation of spo11-1 mutants of A.thaliana To identify mutants affected in meiosis, we screened the Versailles collection of T-DNA-transformed lines (Bechtold et al., 1993) as well as ethyl-methane sulfonate (EMS)-mutagenized lines for reduced fertility. Screening for sterility among mutagenized plants allowed two allelic mutations conferring a semi-sterile phenotype (Figure 1) to be isolated. Figure 1.spo11-1-1 mutant phenotype. Comparison of 6-week-old Ws (left) and spo11-1-1 (right) plants. Higher magnification of one inflorescence of each is also presented to focus on fruit development (arrows). The scale bar represents 3 cm. Download figure Download PowerPoint The semi-sterile phenotype segregated 3:1 as a single recessive mutation in both lines (see Materials and methods). According to the results shown later, the T-DNA and EMS alleles were called spo11-1-1 and spo11-1-2, respectively. Male and female fertilities are affected in spo11-1 mutants Plants heterozygous for one of the spo11-1 mutations could not be distinguished from wild type. Homozygous spo11-1 mutant plants exhibited an overall morphology similar to wild type, except for seed production (Figure 1). Both mutant alleles produced very reduced amounts of seeds (on average 2 seeds per silique instead of 45 ± 5 for wild-type plants). Furthermore, 32% of these seeds were abnormal (dark, shrunken and non-viable). Seeds counted on spo11-1 mutants come from self-fertilization of the plants. In order to examine the effects of spo11-1 mutation on male and female fertilities individually, a spo11-1-1 homozygous mutant plant was used as either the female or the male parent in crosses with a wild-type strain of the same line [ecotype Wassilewskija (Ws)]. When the mutant was used as the female parent, an average of 3.2 seeds per silique were recovered (counted from 51 siliques) and among them very few abnormal seeds were observed (0.2 per silique). When reciprocal crosses were performed (using the spo11-1-1 mutant as male parent), an average of 10.1 seeds per silique were obtained (counted from 46 siliques). Among these, 33% were abnormal (dark and shrunken). These data show that abnormal seeds observed in selfing progeny probably come from male gamete defects. Furthermore, these crosses indicate that both male and female fertilities are impaired by the spo11-1-1 mutation, explaining the low level of seed production observed in spo11-1 selfing progeny. To investigate the basis of these gametic defects, we examined mature gametophytes in mutant plants (Figure 2). The life cycle of flowering plants is predominantly diploid (sporophytic). The production of gametes occurs within haploid gametophytes (male pollen grains and female embryo sacs) differentiated within the sporophyte after meiosis. Figure 2.Male and female gametophyte observation. Viability of mature pollen grains of Ws (A) or spo11-1-1 (B) plants after Alexander staining. Cytoplasm from viable pollen grains is coloured pink. Arrows point to dead pollen grains. The scale bar represents 25 μm in (A) and (B). (C–F) DIC observation of mature ovules of Ws (C) or spo11-1 plants (D–F) after clearing. In a wild-type mature ovule (C), we can observe some of the seven cells of a mature embryo sac (ec, egg cell; cc, central cell; ac, antipodal cells; synergids are out of focus). At the same stage of development, we observe various differentiation stages in the mutant. As examples, in (D) no embryo sac has developed and only degenerated cells can be seen (arrow), (E) shows a one-nucleus embryo sac and (F) shows a four-nuclei embryo sac. For (C–F), the scale bar represents 35 μm. Download figure Download PowerPoint Mature pollen grains scored for viability by Alexander staining (Figure 2A and B) showed that spo11-1 mutants produced a large proportion of dead pollen in their anthers (shown by arrows for spo11-1-1 in Figure 2B) when compared with wild type (Figure 2A), where 100% of the mature pollen was stained pink. Furthermore, remaining ‘viable’ pollen grains in mutants (monitored by the colouration of the cytoplasm) were variable in size and shape. In vitro germination of spo11-1-1 pollen showed that ∼11% of the pollen grains (among 2969 investigated) germinated, whereas 97% germination of wild-type pollen was observed (data not shown). On the female side, embryo sacs (mature female gametophytes) develop within ovules. In every ovule, a single nucellar cell differentiates into the megaspore mother cell and undergoes meiosis to produce a tetrad of haploid megaspores. Only one of these survives, and after three mitotic divisions gives rise to an eight-nuclei coenocytic embryo sac, which will then cellularize into a seven-cell embryo sac (Figure 2C). As the embryo sac develops, surrounding ovule tissues (nucellus, integuments and funiculus) evolve to produce a fully curved (anatrope) mature ovule (for a review see Schneitz et al., 1995). Wild-type 2-mm pistils were cleared and observed by differential interference contrast microscopy (DIC). The mature embryo sac composed of seven mature cells could be observed (Figure 2C). In spo11-1-1 plants, however, only 3% (among 1358 observed) of the ovules showed such differentiated female gametophytes. In the remaining ovules, we observed fully developed ovules with no distinguishable embryo sac in 74% of the cases (Figure 2D). However, some intermediate stages of development were sometimes observed, as shown in Figure 2E and F, where apparently mature ovules (as defined by the anatrope shape) contain embryo sacs with one, two or four nuclei, suggesting that meiosis occurred but differentiation of the embryo sac has stopped at different stages of development. Data are shown for spo11-1-1, but were also observed for the spo11-1-2 allele. Both male and female meiosis are altered in spo11-1 mutants To further determine what stages of sporogenesis or gametogenesis are impaired in spo11-1 mutants, we observed the developing male and female gametophytes by DIC after clearing treatments. Comparison of early steps of male sporogenesis did not reveal any difference between wild-type and mutant plants (Figure 3A and B): round pollen mother cells (PMCs) could be distinguished within the anther loculus. Later, when these cells had performed the two meiotic divisions, we could observe, in wild-type anthers, the characteristic tetrad of microspores encased in a callose wall (Figure 3C). In mutant plants (data are shown only for spo11-1-1), meiosis products encased in a callose wall were observed (Figure 3D), but instead of a regular tetrahedral structure, either asymmetric tetrads or ‘polyads’ containing more than four meiotic products could be distinguished. Figure 3.DIC observation of developing pollen grains. (A and B) PMCs at meiosis. Round meiocytes can be seen in wild-type anthers (A) as well as in mutant ones (shown for spo11-1-1; B). (C and D) After meiosis, microspores encased in callose are visible in anthers. The four regular products of meiosis arranged in tetrahedra are observed in wild type (C), whereas irregular tetrads and polyads containing more than four cells are seen in the mutant (D). Scale bar = 10 μm. Download figure Download PowerPoint Male meiosis was therefore investigated, staining chromosomes with 4′,6-diamidino-2-phenylindole (DAPI) (Figures 4, 5, 7 and 8). Wild-type Arabidopsis meiosis has already been described in detail by Ross et al. (1996) and Figure 4 summarizes its major stages. At metaphase I, the five bivalents of Arabidopsis are easily distinguishable (Figure 4A), each of them being composed of two homologous chromosomes linked together by chiasmata (see also Figure 7F). Anaphase I leads to the formation of dyads containing two pools of five chromosomes (Figure 4B). A partial decondensation of the chromosomes (Figure 4C) occurs before meiosis II, during which segregation of sister chromatids happens, producing four pools of five chromosomes (Figure 4D). Decondensation of chromosomes (Figure 4E) and cytokinesis then take place, producing tetrads of microspores encased in callose (Figures 4F and 3C). Figure 4.DAPI staining of wild-type (Ws) PMCs during meiosis. The five bivalents of homologous chromosomes are located at the centre of the cell at metaphase I (A). After the first anaphase, two pools of five chromosomes can be observed (B). They will partially decondense (C) before the second meiotic division, which produces four pools of five chromosomes (D). Decondensation of each of them occurs (E) before cytokinesis (F). Scale bar = 10 μm. Download figure Download PowerPoint Figure 5.DAPI staining of spo11-1-1 PMCs during meiosis. At the end of the prophase, cells containing 10 fully condensed chromosomes most of the time are observed (A). When anaphase migration occurs, chromosome bridges can sometimes be seen (B, arrow). Segregation of unpaired chromosomes at anaphase I leads to random partitioning of chromosomes (C–E). The second meiotic division produces cells with more than four pools of DNA, corresponding to a variable number of chromosomes (F). Decondensation (G) and cytokinesis occur, producing polyads (H). Scale bar = 10 μm. Download figure Download PowerPoint Figure 6.Propidium iodide staining of developing ovules at meiosis, observed with a confocal laser scanning microscope. Metaphase I of wild type (A) or spo11-1-1 mutant (C). Metaphase II of wild type (B) or spo11-1-1 mutant (D). Scale bar = 10 μm. Download figure Download PowerPoint Figure 7.DAPI staining of wild-type PMCs during meiotic prophase. (A) Leptotene stage. (B) Partial homologous pairing (arrows). (C and D) Pachytene stages showing fully 'synapsed’ homologous chromosomes. Some bright pericentromeric regions are indicated by arrows. (E) Diplotene stage. (F) Diakinesis stage. Scale bar = 10 μm. Download figure Download PowerPoint Figure 8.DAPI staining of spo11-1-1 PMCs during meiotic prophase. (A) A cell at leptotene. In (B and C), chromosomes are condensing but no formation of bivalents is observed. (D) Diakinesis. Scale bar = 10 μm. Download figure Download PowerPoint In spo11-1 mutant PMCs, the 10 Arabidopsis chromosomes were condensed, but bivalents were rarely formed (Figure 5A). However, some chromosome bridges were observed (Figure 5B, arrow), showing that chromosomes can still sometimes form bivalents. Out of 84 spo11-1 cells in metaphase I or in the beginning of anaphase I, 36 contained chromosomes showing evidence of some cross-over (39 chromosome bridges and 8 bivalents). These data suggest that ∼7% of chiasma are formed compared with wild type since an average of nine cross-overs per wild-type meiosis was reported by Copenhaver et al. (1998). Anaphase I proceeded, but because most homologous chromosomes were not linked together by chiasmata, segregation appeared to be random. Instead of the two pools of five chromosomes observed at metaphase II in wild-type cells (Figure 4B), variable partitioning of the 10 chromosomes was seen in mutant meiocytes (Figure 5C, D and E). Sister chromatids were then separated and cells with 20 chromosomes were observed (Figure 5F). Decondensation (Figure 5G) and cytokinesis occurred normally, producing the ‘polyads’ already described (Figure 3D) with variable number of cells (Figure 5H), reflecting the variation in numbers of pools of chromosomes obtained at the end of meiosis. Female meiosis was also investigated after chromosome staining by propidium iodide and observation by confocal microscopy. Univalents were also observed at the end of prophase I (compare Figure 6C and A), leading to random segregation of the chromosomes at anaphase I (compare Figure 6B and D). Therefore, gametophyte development deficiencies described earlier appeared to be a consequence of aberrant meiosis in the mutants. Chromosome behaviour during the earlier stage of meiosis was then analysed, comparing meiotic prophase I in wild type (Figure 7) and in the spo11-1-1 mutant (Figure 8). Figure 7A shows a leptotene nucleus with very thin chromosome threads. Later, synapsis progresses and can be identified in some nuclei where loops of paired and unpaired chromosomes alternate (Figure 7B, arrows). Synapsis is maximum at pachytene (Figure 7C and D), during which bivalents appear thick. Later, each member of the bivalent tends to separate (Figure 7E), and chiasmata become visible at diakinesis (Figure 7F). Out of 90 cells in prophase I in wild type, we found six in leptotene (Figure 7A), 21 in diakinesis (Figure 7F) and 43 in pachytene (Figure 7C and D). The remaining cells (20) showed intermediate synapsis. The same analyses on the spo11-1-1 mutant did not allow the identification of any pachytene stage among 160 cells observed. Nevertheless, condensation progressed until diakinesis [eight cells were observed in leptotene (Figure 8A), 27 in diakinesis (Figure 8D) and 125 at intermediate stages of condensation like the one shown in Figure 8B and C], but fully synapsed chromosomes were never observed. Molecular characterization of spo11-1 mutations Plant DNA flanking the T-DNA insertion in the spo11-1-1 allele was cloned either by plasmid rescue or tail PCR. PCR mapping on the CIC YAC library showed that the two borders map on the same part of chromosome 3 on CIC clones 6H6 and 5G8. The Columbia allele of this gene was found in the database as part of a P1 clone sequenced on chromosome three of Arabidopsis (clone MJG9, DDBJ/EMBL/GenBank accession No. AP000375). The T-DNA insertion occurred between nucleotides 43553 and 43555. The EMS mutation (spo11-1-2) was mapped to chromosome 3, between morphological markers hy2 (12 cM) and gl1 (30 cM), confirming the location of spo11-1 mutations on the top arm of chromosome 3 between 15 and 25 cM. Southern analyses (not shown) showed that spo11-1-1 possessed two linked left borders (LB) and no right border (RB). Indeed, cloning and sequencing of the T-DNA flanking sequences showed that a recombined insertion had occurred (Figure 9). A first LB was truncated within the 24 bp repeat, which is observed frequently in T-DNA transformants (LB1; Figure 9). The RB region and the promotorless GUS coding sequence of the T-DNA were deleted, and replaced by a piece of the binary vector pGKB5 and a second LB, complementary to the first one (LB2; Figure 9). Figure 9.AtSPO11-1 coding region and spo11-1 mutations. Schematic representations of AtSPO11-1, spo11-1-1 T-DNA insertion and pGKB5 transformation vector. Black boxes represent exons [from the start of translation (ATG) to the stop codon (STOP)] of the main transcript of the AtSPO11-1 gene. The location of the T-DNA insertion in the spo11-1-1 allele as well as the location of the point mutation that occurred in the spo11-1-2 allele are indicated by vertical arrows. The pGKB5 transformation vector used to generate the T-DNA transformant collection and the recombined T-DNA structure of the spo11-1-1 allele are indicated underneath. Boxes represent the T-DNA borders (RB and LB), where the 24 bp repeats are indicated in black. The orientation of the T-DNA coding sequences (bar gene conferring basta resistance, nptII gene conferring kanamycin resistance and uidA gene encoding the GUS enzyme) is indicated by horizontal arrows. Download figure Download PowerPoint We found that T-DNA insertion occurred in the first exon of AtSPO11-1 recently described by Hartung and Puchta (2000). T-DNA insertion in the spo11-1-1 allele disrupted the gene as early as the first exon (Figures 9 and 10), probably leading to a null mutant. The Ws sequence for AtSPO11-1 was obtained (DDBJ/EMBL/GenBank accession No. AF302928) and compared with the Columbia sequence, showing 96% identity at the nucleotide level. cDNA was obtained through RT–PCR (data not shown) and encodes a protein showing 98.6% identity with the Columbia sequence (Figure 10). Expression studies performed by RT–PCR showed that AtSPO11-1 is expressed at a low level and mainly, but not exclusively, in reproductive tissues (data not shown). Interestingly, alternate splicing as described previously by Hartung and Puchta (2000) was constantly observed. It is not clear yet how this pattern of intron retention is developmentally regulated. Sequencing of the AtSPO11-1 gene in the EMS allele (spo11-1-2) revealed a C to T transition in the second exon of AtSPO11-1, transforming a glutamine codon CAA into a stop codon TAA (Figures 9 and 10), confirming that mutant phenotypes observed in both mutants are the result of a non-functional AtSPO11-1 gene. Figure 10.Clustal_X alignment of the three Arabidopsis SPO11-like proteins (Columbia ecotype) (AtSPO11-1, DDBJ/EMBL/GenBank accession No. CAB81544; AtSPO11-2, accession No. CAB81545; AtSPO11-3, accession No. CAB86036) with S.cerevisiae (Sc; accession No. AAA65532) and mouse (accession No. AAD52563) SPO11 proteins as well as S.shibatae topoisomerase VI A protein (accession No. O05208). Conserved motifs I–V, as defined by Bergerat et al. (1997), are indicated in black boxes and conserved amino acids are shadowed in grey. The location of the T-DNA insertion in the spo11-1-1 allele and the stop mutation of the spo11-1-2 allele are indicated by arrowheads. An asterisk shows the conserved tyrosine (in position 135 in S.cerevisiae) thought to be responsible for DNA cleavage (Bergerat et al., 1997). Amino acids that differ between Ws and Columbia AtSPO11-1 are underlined. An isoleucine replaces the phenylalanine (position 5), a glutamic acid replaces the valine (position 26), a serine replaces the asparagine (position 39), a leucine replaces the phenylalanine (position 65), and there is an insertion of a valine in position 213 between the underlined threonine and glycine. Download figure Download PowerPoint Hartung and Puchta (2000) described a second Arabidopsis SPO11-like gene located on chromosome 1 (DDBJ/EMBL/GenBank accession No. AJ251990). In addition, a third sequence putatively encoding a protein with strong similarity to Spo11 was detected in the Arabidopsis genome (chromosome 5, DDBJ/EMBL/GenBank accession Nos AL162973, CAB86036). We checked that this third gene is transcribed (DDBJ/EMBL/GenBank accession number No. AF323679). Figure 10 shows an alignment of several SPO11/Topo VI A orthologues including the three Arabidopsis homologues. The level of amino acid identity between AtSPO11-1 and S.cerevisiae Spo11 is 30%. Conserved regions I–V, as defined by Bergerat et al. (1997), are indicated. Region I contains the active tyrosine site (indicated by an asterisk), which is invariant among all the proteins of this family and which has been shown to be necessary for DSB induction in S.cerevisiae (Bergerat et al., 1997). AtSPO11-1 disruption drastically decreases meiotic recombination The S.cerevisiae Spo11 protein is thought to catalyse DNA DSBs that initiate meiotic recombination. To examine recombination frequencies in a spo11-1 mutant background, lines heterozygous for the spo11-1-1 mutation (Ws ecotype) were crossed to Columbia (Col-0). Meiotic recombination rates were investigated in the progeny of F2 plants either homozygous for the mutation spo11-1-1, heterozygous or wild type. Microsatellites showing polymorphisms between the two ecotypes used (Bell and Ecker, 1994) were chosen to measure recombination frequencies on two chromosome arms: the bottom of chromosome 1 between markers nga280 and nga111, and the top of chromosome V between markers nga106 and nga76. MAPMAKER (Lander et al., 1987) was used to convert data into genetic distance in centimorgans (Table I). According to the recent RI map (http://nasc.nott.ac.uk/), markers nga280 and nga111 are separated by 31.72 cM, and markers nga76 and nga106 by 35.05 cM. These data fit with the distances we calculated from our results on either heterozygous or wild-type plants (Table I; spo11-1/SPO11-1 and SPO11-1/SPO11-1 plants). In contrast, distances between the same markers calculated in the spo11-1 background were ∼10 times lower, since the calculated map distance between nga111 and nga280 has fallen from 31.72 to 4.3 cM, and the distance between nga76 and 106 from 35.05 to 2.4 cM. We checked that this effect is not plant dependent since three different homozygous spo11-1/spo11-1 plants were used to collect these data (sister plants 1
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