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Identification of alpha atrial natriuretic peptide [4–28] and [5–28] in porcine brain

1987; Elsevier BV; Volume: 149; Issue: 3 Linguagem: Inglês

10.1016/0006-291x(87)90515-8

1090-2104

Shuzo Ueda, Tetsuji Sudoh, Kenji Fukuda, Kenji Kangawa, Naoto Minamino, Hisayuki Matsuo,

Cardiac electrophysiology and arrhythmias

Although atrial natriuretic peptide (ANP) has recently been verified to function as a neuropeptide in the central nervous system, its definite identification has not been done so far. We have isolated two ANP-related peptides from porcine brain by utilizing α-ANP specific radioimmunoassay coupled with immunoaffinity chromatography and reverse phase HPLC. By structual analyses, these two peptides were determined to be α-ANP[4–28] and α-ANP[5–28]. They were found to elicit chick rectum relaxant activity comparable to α-ANP. These results indicate that proteolytic processing of ANP precursor in the central nervous system takes place in a manner different from that in heart and plasma, where γ-ANP and α-ANP are known to be the main components of ANP, respectively.