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Biochemical characterisation of flagellates isolated from fruits and seeds from Brazil

1999; Oxford University Press; Volume: 170; Issue: 2 Linguagem: Inglês

10.1111/j.1574-6968.1999.tb13393.x

1574-6968

C Fernández-Ramos, F. Javier Luque, Carmen Fernández-Becerra, Antonio Osuna, Shiduca Itow Jankevicius, José Vítor Jankevicius, María J. Rosales, Manuel Sánchez‐Moreno,

Nematode management and characterization studies

Seven flagellates of the family Trypanosomatidae have been isolated from different fruits and seeds from Brazil, three from tomato fruit (Lycopersicum esculentum), one from urucum fruit (Bixa orellana), two from maize kernels (Zea mays) and one from bean (Phaseolus vulgaris). These isolates were characterised by isoenzymatic analysis, using nine enzymatic systems and the isoenzymatic profiles were compared with those obtained from different flagellates already described as belonging to the genera Phytomonas, Herpetomonas and Crithidia. Most of the isoenzymes were able to distinguish the new plant isolates from insect trypanosomatids (Herpetomonas and Crithidia). Six of the isolates could be ascribed to the genus Phytomonas, five of them being phylogenetically closely related to Phytomonas serpens. The new isolates excreted the same metabolic end-products into the external medium and only small quantitative differences were found among the different isolates.