A clinical grade cocktail of cytokines and PGE2 results in uniform maturation of human monocyte-derived dendritic cells: implications for immunotherapy
2002; Elsevier BV; Volume: 20; Linguagem: Inglês
10.1016/s0264-410x(02)00382-1
ISSN1873-2518
AutoresAndrew W. Lee, Tuan Truong, Kara Bickham, Jean‐François Fonteneau, Marie Larsson, Ida Da Silva, Selin Somersan, Elaine K. Thomas, Nina Bhardwaj,
Tópico(s)RNA Interference and Gene Delivery
ResumoDendritic cells (DCs) can induce tumor- or pathogen-specific T cell responses in humans. We comprehensively compared the clinically available DC maturation stimuli for their ability to promote uniformly mature DCs that elicit higher levels of T cell responses. We compared the standard maturation stimulus, autologous monocyte-conditioned medium (MCM), with a synthetic double stranded RNA (poly I:C), soluble CD40 ligand trimer, and a defined cocktail of cytokines (TNF-α, IL-1β, IL-6) and PGE2 to promote mature phenotype and function in human monocyte-derived DCs. The cocktail was the most efficient despite the lack of induction of IL-12p70. While these results support the use of the MCM-mimic cocktail in clinical DC immunotherapy trials, the roles of it's individual constituents remain to be completely defined.
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