Produção Nacional
Revisado por pares
Detection of Benz[ j ]aceanthrylene in Urban Air and Evaluation of Its Genotoxic Potential
2015; American Chemical Society; Volume: 49; Issue: 5 Linguagem: Inglês
10.1021/es505458g
ISSN1520-5851
AutoresHwanmi Lim, Åse Mattsson, Ian Jarvis, Christoffer Bergvall, Matteo Bottai, Daniel Alexandre Morales, Fábio Kummrow, Gisela de Aragão Umbuzeiro, Ulla Stenius, Roger Westerholm, Kristian Dreij,
Tópico(s)Genetically Modified Organisms Research
ResumoBenz[j]aceanthrylene (B[j]A) is a cyclopenta-fused polycyclic aromatic hydrocarbon with strong mutagenic and carcinogenic effects. We have identified B[j]A in air particulate matter (PM) in samples collected in Stockholm, Sweden and in Limeira, Brazil using LC–GC/MS analysis. Determined concentrations ranged between 1.57 and 12.7 and 19.6–30.2 pg/m3 in Stockholm and Limeira, respectively, which was 11–30 times less than benzo[a]pyrene (B[a]P) concentrations. Activation of the DNA damage response was evaluated after exposure to B[j]A in HepG2 cells in comparison to B[a]P. We found that significantly lower concentrations of B[j]A were needed for an effect on cell viability compared to B[a]P, and equimolar exposure resulted in significant more DNA damage with B[j]A. Additionally, levels of γH2AX, pChk1, p53, pp53, and p21 proteins were higher in response to B[j]A than B[a]P. On the basis of dose response induction of pChk1 and γH2AX, B[j]A potency was 12.5- and 33.3-fold higher than B[a]P, respectively. Although B[j]A levels in air were low, including B[j]A in the estimation of excess lifetime cancer risk increased the risk up to 2-fold depending on which potency factor for B[j]A was applied. Together, our results show that B[j]A could be an important contributor to the cancer risk of air PM.
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