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Limit of Detection, Dilution Factors, and Technique Compatibility in Multidimensional Separations Utilizing Chromatography, Capillary Electrophoresis, and Field-Flow Fractionation

1999; American Chemical Society; Volume: 71; Issue: 8 Linguagem: Inglês

10.1021/ac981128q

1520-6882

Mark R. Schure,

Crystallization and Solubility Studies

The theoretical development of the limit of detection (LOD) concept is extended from one separation column or channel used in gas, liquid, and size-exclusion chromatography, capillary electrophoresis, and field-flow fractionation to the case where multiple columns and channels are utilized in the so-called "comprehensive" and "heart cutting" modes of operation. Simple equations show that the column dilution factors and the split ratios used to compute the LOD are multiplicative per dimension. Thus, when more than one separation dimension is utilized in a sequential column mode of operation, a larger overall dilution of the original injected solute concentration will occur. The dilution of the initial zone in multidimensional geometrical separation systems such as planar two-dimensional thin-layer chromatography and two-dimensional slab gel electrophoresis is also considered. The practical aspects of this dilution are discussed with respect to the types of separation techniques that can be used to implement multidimensional separation systems.