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Promotion of Development of Bovine Embryos Produced In Vitro by Addition of Cysteine and β-Mercaptoethanol to a Chemically Defined Culture System

1998; Elsevier BV; Volume: 81; Issue: 2 Linguagem: Inglês

10.3168/jds.s0022-0302(98)75586-9

1529-9066

J. N. Caamaño, Zae Young Ryoo, Curtis R. Youngs,

Animal Genetics and Reproduction

The aim of this research was to determine the effects of L-cysteine and beta-mercaptoethanol on the in vitro development of bovine embryos that had been produced in vitro. A 2 x 3 factorial arrangement of treatments was used to evaluate the effect of 0.63 or 6.9 microM L-cysteine and 0, 10, or 100 microM beta-mercaptoethanol on the development of bovine embryos in a chemically defined medium. Embryos containing 6 to 8 cells were randomly allocated to one of the six treatment combinations and were cultured for 7 d. Both beta-mercaptoethanol and L-cysteine increased the number of embryos that reached the blastocyst stage of development, although no interaction was observed between the compounds. Embryos that were cultured in the presence of beta-mercaptoethanol had more cells at the blastocyst stage than did embryos cultured in medium without beta-mercaptoethanol. These findings provide evidence that beta-mercaptoethanol and L-cysteine promote increased embryonic development and that beta-mercaptoethanol increases the number of cells in bovine embryos produced in vitro and cultured in a cell-free, protein-free culture system.