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Type-specific collagenolysis: A type V collagen-degrading enzyme from macrophages

1980; Elsevier BV; Volume: 97; Issue: 3 Linguagem: Inglês

10.1016/0006-291x(80)91490-4

1090-2104

Carlo L. Mainardi, Jerome M. Seyer, Andrew H. Kang,

Cell Adhesion Molecules Research

An enzymatic activity capable of degrading type V collagen at neutral pH was found in the medium from cultured rabbit pulmonary alveolar macrophages which had been "activated" invivo by injection of complete Freund's Adjuvant. This enzyme was characterized as a metalloproteinase by virtue of its inhibition by EDTA but not by phenylmethylsulfonyl fluoride or N-ethyl maleimide. Ion-exchange chromatography on DEAE-cellulose was successful in separating the type V collagen-degrading activity from the type I collagenase which is also secreted by these cells. These observations suggest that the degradation of type V collagen is independent of the degradation of the interstitial collagens and may require the action of its own "specific collagenase".