Incorporation of 5‐Iodocytidine into Yeast tRNA Phe with tRNA Nucleotidyl Transferase in vitro
1972; Wiley; Volume: 25; Issue: 2 Linguagem: Inglês
10.1111/j.1432-1033.1972.tb01692.x
ISSN1432-1033
AutoresMathias Sprinzl, Friedrich von der Haar, E. Schlimme, Hans Sternbach, Friedrich Cramer,
Tópico(s)RNA Research and Splicing
Resumo5‐Iodocytidine (io 5 C) is incorporated into tRNA Phe ‐A 73 as well as into tRNA Phe ‐A 73 ‐C 74 using tRNA nucleotidyl transferase. The tRNA Phe ‐A 73 ‐C 74 ‐io 5 C 75 ‐A 76 and tRNA Phe ‐A 73 ‐io 5 C 74 ‐io 5 C 75 ‐A 76 , respectively, thus obtained can be aminoacylated by the corresponding synthetase exhibiting the same K m but a slightly lowered V compared to tRNA Phe ‐A 73 ‐C 74 ‐C 75 ‐A 76 . An analytical method is presented which enables quantitative determination of the 3′ terminal nucleosides.
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