Precursor RNAs Harboring Nonsense Codons Accumulate Near the Site of Transcription
2001; Elsevier BV; Volume: 8; Issue: 1 Linguagem: Inglês
10.1016/s1097-2765(01)00288-x
ISSN1097-4164
AutoresOliver Mühlemann, Caroline S Mock-Casagrande, Jun Wang, Shulin Li, Noélia Custódio, Maria Carmo‐Fonseca, Miles Wilkinson, Melissa J. Moore,
Tópico(s)RNA modifications and cancer
ResumoMessenger RNAs containing premature termination codons (PTCs) are selectively eliminated by nonsense-mediated mRNA decay (NMD). Paradoxically, although cytoplasmic ribosomes are the only known species capable of PTC recognition, in mammals many PTC-containing mRNAs are apparently eliminated prior to release from the nucleus. To determine whether PTCs can influence events within the nucleus proper, we studied the immunoglobulin (Ig)-μ and T cell receptor (TCR)-β genes using fluorescent in situ hybridization (FISH). Alleles containing PTCs, but not those containing a missense mutation or a frameshift followed by frame-correcting mutations, exhibited elevated levels of pre-mRNA, which accumulated at or near the site of transcription. Our data indicate that mRNA reading frame can influence events at or near the site of gene transcription.
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