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Specific chemical labeling of DNA fragments

1979; Oxford University Press; Volume: 7; Issue: 6 Linguagem: Inglês

10.1093/nar/7.6.1485

1362-4962

Hilbert Eshaghpour, Dieter Söll, D M Crothers,

Click Chemistry and Applications

We describe a simple method for specific chemical labeling of DNA fragments at their 3'-termin1. The procedure includes enzymatic addition of 4-th1ourid1ne, followed by reaction in mild non-denaturing conditions with the highly reactive α-haloacetamido derivatives of several chemical labels. The attached reporter molecule can be removed by extended treatment with β-mercaptoethanol. Among the potential applications of this labeling method is the study of specific protein-DNA interactions in solution.