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An Assessment of Software Solutions for the Analysis of Mass Spectrometry Based Quantitative Proteomics Data

2008; American Chemical Society; Volume: 7; Issue: 1 Linguagem: Inglês

10.1021/pr700758r

1535-3907

Lukas Mueller, Mi‐Youn Brusniak, D.R. Mani, Ruedi Aebersold,

Metabolomics and Mass Spectrometry Studies

Over the past decade, a series of experimental strategies for mass spectrometry based quantitative proteomics and corresponding computational methodology for the processing of the resulting data have been generated. We provide here an overview of the main quantification principles and available software solutions for the analysis of data generated by liquid chromatography coupled to mass spectrometry (LC-MS). Three conceptually different methods to perform quantitative LC-MS experiments have been introduced. In the first, quantification is achieved by spectral counting, in the second via differential stable isotopic labeling, and in the third by using the ion current in label-free LC-MS measurements. We discuss here advantages and challenges of each quantification approach and assess available software solutions with respect to their instrument compatibility and processing functionality. This review therefore serves as a starting point for researchers to choose an appropriate software solution for quantitative proteomic experiments based on their experimental and analytical requirements.