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Creation of human cardiac cell sheets using pluripotent stem cells

2012; Elsevier BV; Volume: 425; Issue: 2 Linguagem: Inglês

10.1016/j.bbrc.2012.07.089

1090-2104

Katsuhisa Matsuura, M. Wada, Tatsuya Shimizu, Yuji Haraguchi, Fumiko Sato, Kasumi Sugiyama, Kanako Konishi, Yuji Shiba, Hinako Ichikawa, Aki Tachibana, Uichi Ikeda, Masayuki Yamato, Nobuhisa Hagiwara, Teruo Okano,

3D Printing in Biomedical Research

Although we previously reported the development of cell-dense thickened cardiac tissue by repeated transplantation-based vascularization of neonatal rat cardiac cell sheets, the cell sources for human cardiac cells sheets and their functions have not been fully elucidated. In this study, we developed a bioreactor to expand and induce cardiac differentiation of human induced pluripotent stem cells (hiPSCs). Bioreactor culture for 14 days produced around 8 × 107 cells/100 ml vessel and about 80% of cells were positive for cardiac troponin T. After cardiac differentiation, cardiomyocytes were cultured on temperature-responsive culture dishes and showed spontaneous and synchronous beating, even after cell sheets were detached from culture dishes. Furthermore, extracellular action potential propagation was observed between cell sheets when two cardiac cell sheets were partially overlaid. These findings suggest that cardiac cell sheets formed by hiPSC-derived cardiomyocytes might have sufficient properties for the creation of thickened cardiac tissue.