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Effects of γ‐alumina and hydroxyapatite coatings on the growth and metabolism of human osteoblasts

1995; Wiley; Volume: 29; Issue: 11 Linguagem: Inglês

10.1002/jbm.820291111

1097-4636

Béatrice Labat, Annette Chamson, Jacques Frey,

Orthopaedic implants and arthroplasty

Abstract The behavior of human osteoblasts cultivated on hydroxyapatite or alumina‐coated disks of Ti6AL4V was studied in vitro. Cell anchorage and spreading were observed by scanning electron microscopy. Cell growth was monitored by counting cells and measuring DNA at 5 h and 2, 5, and 10 days after cell seeding. Cells grown for 10 days were labeled with 14 C‐proline and total protein and collagen synthesis were measured; the type of collagen was also determined. Both ceramics showed excellent biocompatibility. At 10 days of culture the cells showed a higher rate of proliferation on alumina than on hydroxyapatite. Neither ceramic altered the collagen biosynthesis or the osteoblastlike properties of the cells, as indicated by the high percentage of type I collagen. © 1995 John Wiley & Sons, Inc.