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Highly enantioselective bioreduction of ethyl 3-oxohexanoate

2011; Elsevier BV; Volume: 52; Issue: 46 Linguagem: Inglês

10.1016/j.tetlet.2011.09.023

1873-3581

Aline de Souza Ramos, Joyce Benzaquem Ribeiro, Raquel de Oliveira Lopes, Selma Gomes Ferreira Leite, Rodrigo O. M. A. de Souza,

Biofuel production and bioconversion

Seven wild-type microorganism strains were used to reduce ethyl 3-oxohexanoate to ethyl (R)-3-hydroxyhexanoate. Free cells of Kluyveromyces marxianus and Aspergillus niger led to higher than 99% of conversion with higher than 99% ee. After immobilization in calcium alginate spheres, cells of K. marxianus exhibited high enantioselectivity (>99% ee) and conversion level (99%) within 24 h even if substrate was added at concentration of 10 g/L (62 mM).