Detection of white-rot fungi by a non-toxic stain
1990; Cambridge University Press; Volume: 94; Issue: 1 Linguagem: Inglês
10.1016/s0953-7562(09)81260-4
ISSN1469-8102
AutoresM-L. Niku-Paavola, Laura Raaska, Merja Itävaara,
Tópico(s)melanin and skin pigmentation
ResumoA method is described for distinguishing between white-rot fungi producing laccase (EC 1 · 10 · 3 · 2) and brown-rot fungi producing catechol oxidase (EC 1 · 10 · 3 · 1). Laccase oxidizes a redox indicator ABTS (2, 2′-azino-di(3-ethylbenzothiazoline-6-sulfonic acid)) to form a blue-green cation radical whilst catechol oxidase does not. The method is suitable for detecting laccase excreted by mycelia growing on agar and wood-based substrates, and proteins derived from culture liquids after electrophoretic separation on polyacrylamide gel. The method can be adapted for quantitative estimation by spectrophotometry.
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