Immunocytochemical localization of GABA neurons in the rabbit and frog retina
1980; Elsevier BV; Volume: 5; Linguagem: Inglês
10.1016/0361-9230(80)90004-0
ISSN1873-2747
AutoresChristopher Brandon, Dominic Man-Kit Lam, Y. Y. Thomas Su, Jang‐Yen Wu,
Tópico(s)Lipid Membrane Structure and Behavior
ResumoThe visualization of γ-aminobutyric acid (GABA) neurons in rabbit and frog retinas has been carried out, using an immunocytochemical technique for the localization of the GABA-synthesizing enzyme glutamic acid decarboxylase (GAD). In the rabbit, immunoreactivity was restricted to a small group of amacrine cell bodies and their laminated processes in the inner plexiform layer. Electron microscopic examination showed that these processes were presynaptic to ganglion cell dendrites (infrequently), amacrine cell telodendrons, and bipolar cell terminals. Often, bipolar cell terminals were found which were densely innervated by several GAD-positive processes. No definite synapses were observed in which a GAD-positive process represented the postsynaptic element. In the frog, dense GAD immunoreactivity was observed in the inner plexiform layer, both as punctate deposits and as filled processes of stratified and diffuse amacrine cells; in the inner nuclear layer, where many cell bodies were labeled, including those of some horizontal cells; and diffusely in the outer plexiform layer.
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