Structural characterization and DPPH· radical scavenging activity of a polysaccharide from Guara fruits
2013; Elsevier BV; Volume: 103; Linguagem: Inglês
10.1016/j.carbpol.2013.12.009
ISSN1879-1344
AutoresDehong Hua, Dezhi Zhang, Bing Huang, Pan Yi, Chunyan Yan,
Tópico(s)Plant Physiology and Cultivation Studies
ResumoThe crude polysaccharides were extracted from fruits of Psidium guajava Linn. by hot water. After removal of proteins, isolation and purification by DEAE-52 Cellulose chromatography and Sephadex G-75 gel filtration, a polysaccharide (GP70-2) was obtained and structurally characterized. GP70-2 has a relative molecular weight of 74 kDa and was composed of D-galactose and L-arabinose in the ratio of 1:1, with a specific optical rotation of [a]D(25) = +101°. Structural characterization of this novel polysaccharide was carried out using infrared spectroscopy, methylation analyses, and NMR studies ((1)H, (13)C, (1)H-(1)H-COSY, HMQC, and HMBC). Based on the above data, the following structure was assigned to the repeated core unit of GP70-2: [Formula: see text]. This polysaccharide showed a concentration dependent DPPH· radical scavenging activity.
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