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Difference in number of loci of swine leukocyte antigen classical class I genes among haplotypes

2008; Elsevier BV; Volume: 93; Issue: 3 Linguagem: Inglês

10.1016/j.ygeno.2008.10.004

1089-8646

Maiko Tanaka-Matsuda, Asako Ando, Claire Rogel Gaillard, Patrick Chardon, Hirohide Uenishi,

Immune Cell Function and Interaction

The structure of the entire genomic region of swine leukocyte antigen (SLA)—the porcine major histocompatibility complex—was recently elucidated in a particular haplotype named Hp-1.0 (H01). However, it has been suggested that there are differences in the number of loci of SLA genes, particularly classical class I genes, among haplotypes. To clarify the between-haplotype copy number variance in genes of the SLA region, we sequenced the genomic region carrying SLA classical class I genes on two different haplotypes, revealing increments of up to six in the number of classical class I genes in a single haplotype. All of the SLA-1(-like) (SLA-1 and newly designated SLA-12) and SLA-3 genes detected in the haplotypes thus analyzed were transcribed in the individual. The process by which duplication of SLA classical class I genes was likely to have occurred was interpreted from an analysis of repetitive sequences adjacent to the duplicated class I genes.